Abstract

The selection criterion of subtraction factors used in subtraction microscopy is numerically investigated. The fluorescence peak intensity after subtraction and resolution derivative are proposed as essential parameters for evaluating the subtraction threshold.

© 2015 Optical Society of America

PDF Article

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription