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Quasi-elastic light scattering is a useful method to determine the size distribution of sub-micron particles in fluids. We have applied this technique to measure in vivo changes in the association of human lens protein constituents that occur with aging and cataractogenesis. The autocorrelation function of the scattered light has been analyzed by a two component exponential where each component is characterized by an intensity and decay rate. Each pair of parameters is associated with one of two major protein groups in the lens. These parameters have been determined as a function of age and position within normal, clear lenses.
© 1986 Optical Society of America
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