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Conferences > LACA > 1987 > PDP > PDP10

Analysis and Quantitation of Biomolecules by Two-Step Laser Desorption/Multiphoton Ionization Mass Spectrometry

Jong Hoon Hahn, Renato Zenobi, Friedrich Engelke, and Richard N. Zare

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Abstract

We report a new methodology, two-step laser desorption/multiphoton ionization mass spectrometry, for the ultrasensitive detection and quantitation of molecular adsorbates. The method has been successfully applied to a number of nonvolatile and thermally labile compounds, including the 20 primary phenylthiohydantoin (PTH)- amino acids, the 4 DNA bases, the steroid β-estradiol and the large porphyrin, protoporphyrin IX dimethylester.

© 1987 Optical Society of America

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