Combined optical coherence and two-photon excitation microscopy

E. Beaurepaire; L. Moreaux; J. Mertz

doi:10.1364/IVOI.1999.MSI43

Proceedings of Inter-Institute Workshop on In Vivo Optical Imaging at the NIH
Technical Digest Series (Optica Publishing Group, 1999),
paper MSI43
•https://doi.org/10.1364/IVOI.1999.MSI43

Combined optical coherence and two-photon excitation microscopy

E. Beaurepaire, L. Moreaux, and J. Mertz

In Vivo optical Imaging at the NIH 1999

Bethesda, MD United States
16–17 September 1999
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Microscopy and Subsurface Imaging (MSI)

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E. Beaurepaire, L. Moreaux, and J. Mertz, "Combined optical coherence and two-photon excitation microscopy," in Proceedings of Inter-Institute Workshop on In Vivo Optical Imaging at the NIH, Technical Digest Series (Optica Publishing Group, 1999), paper MSI43.

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Abstract

We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited fluorescence (TPEF) microscopy. Independent contrast mechanisms derived from fluorescence and backscattered light provide complementary information on biological tissues. The coupling of OCM and TPEF microscopy is natural in that the two imaging modes may be run off the same laser source. The confocal arrangement for OCM provides a sub-micrometer resolution compatible with TPEF microscopy.

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