Non-Isoplanatic PSF and Image Estimation Using Speckle Illumination

Byungjae Hwang; Rafael Piestun; Kristina Irsch

doi:10.1364/ISA.2023.ITh1C.3

Optica Imaging Congress (3D, COSI, DH, FLatOptics, IS, pcAOP)
Technical Digest Series (Optica Publishing Group, 2023),
paper ITh1C.3
•https://doi.org/10.1364/ISA.2023.ITh1C.3

Non-Isoplanatic PSF and Image Estimation Using Speckle Illumination

Byungjae Hwang, Rafael Piestun, and Kristina Irsch

Imaging Systems and Applications 2023

Boston, Massachusetts United States
14–17 August 2023
ISBN: 978-1-957171-28-9

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B. Hwang, R. Piestun, and K. Irsch, "Non-Isoplanatic PSF and Image Estimation Using Speckle Illumination," in Optica Imaging Congress (3D, COSI, DH, FLatOptics, IS, pcAOP), Technical Digest Series (Optica Publishing Group, 2023), paper ITh1C.3.

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Abstract

We propose a speckle-illumination statistical method to recover the point-spread function (PSF) and high-resolution fluorescence images in the presence of aberrations, without any calibration or isoplanatism assumptions. The accurate PSF estimation enables its use in deconvolution for high-resolution 3D imaging.

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