Abstract

Fluorescence spectroscopy is an extremely useful technique for understanding the complex relationships between structure and function of biological systems. The advantages of fluorescence spectroscopy include sensitivity (only a small amount of sample is needed), a wide range of time scales in which to measure particular processes (picoseconds to seconds), the ability to examine the biological system in aqueous solution, as well as many physical parameters that can be measured (quantum yield, lifetime, emission energies, anisotropy) to reveal different aspects of the system.

© 1988 Optical Society of America