Abstract

Interaction of polarized light with tissue samples is an emergent and powerful tool for biomedical diagnosis due to the possibility of providing quantitative information through Stokes parameters and Mueller matrix elements. Division of Focal Plane polarimetric sensing in particular has recently become popular due to advances in technology and commercial availability of microgrid polarizers integrated onto the sensing chip of a camera. After calibration of the system, intensity measures from these sensors can be mapped to the State of Polarization (SoP) of the input. A microscopy setup incorporating DoFP sensing can allow then to obtain Stokes parameters corresponding to a given Field of View (FoV) of a tissue sample. By illuminating with linearly independent SoP in the input, Mueller matrix elements can also be retrieved for the FoV of the sample. Finally, FoV extension to whole-slide Mueller matrix imaging can be achieved through stitching of multiple images taken by means of a motorized XY stage. Validation results for whole-slide Mueller matrix elements of tissue samples are presented.

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