Abstract
The identification of bacteria is necessary as fast as possible e.g. to provide an appropriate therapy for patients. Here the cultivation time should be kept to a minimum. Beside microbiological identification methods Raman spectroscopy is a valuable tool for bacteria identification. UV-resonance Raman spectroscopy enables selective monitoring of the cellular DNA/RNA content and allows for a genotaxonomic classification of the bacteria. Since UV excitation may lead to sample destruction the measurements are performed on rotated bacterial films.
For a faster identification avoiding the cultivation step single bacteria analysis is necessary. Using micro-Raman spectroscopy a spatial resolution in the size range of the bacteria can be achieved. With this Raman excitation the chemical components of the whole cell are measured which leads to a phenotypic classification. For localization of bacteria inside complex matrices fluorescence labeling is achieved.
© 2007 SPIE
PDF ArticleMore Like This
De Chen, Changan Xie, and Yong-qing Li
CTuEE7 Conference on Lasers and Electro-Optics (CLEO:S&I) 2007
Petra Rösch, Stephan Stöckel, Susann Meisel, Anja Boßecker, Ute Münchberg, Sandra Kloß, Wilm Schumacher, and Jürgen Popp
808708 European Conference on Biomedical Optics (ECBO) 2011
Changan Xie, De Chen, Terrence Hollis, and Yong-qing Li
CTuP2 Conference on Lasers and Electro-Optics (CLEO:S&I) 2005