Abstract

Optical spectroscopy for tumor demarcation was investigated in this study. Laser-induced fluorescence and Raman spectra were measured from normal and tumorous human blood serum in an attempt to discover some values useful in discrimination between normal and tumorous cases. Three Raman peaks were consistently observed from normal blood serum emission using 488.0nm and 514.5nm excitation of an Ar-ion laser, whereas no peak or only slight Raman peaks were detected from tumorous cases. In addition, red shift of fluorescence peak and decrease of fluorescence intensity were observed after samples radiated by laser. According to one thousand twenty-two samples’ spectra, three parameters α, β and Δλ are introduced to distinguish normal, benign and malignant from one another. The application of such parameters in clinical diagnosis was researched. Four hundred and fifty-nine samples were investigated before knowing the result of clinical diagnosis in order to estimate the availability of our criteria.

© 2001 OSA/SPIE