Characterization of the diffusion of fluorophores within microbial biofilms by fluorescence correlation microscopy under two-photon excitation

E. Guiot; M.P. Fontaine-Aupart; R. Briandet; M.N. Bellon-Fontaine; P. Georges; A. Brun; F. Merola; B. Arrio

doi:10.1364/ECBO.2001.4431_8

Photon Migration, Optical Coherence Tomography, and Microscopy
Technical Digest Series (Optica Publishing Group, 2001),
paper 4431_8
•https://doi.org/10.1364/ECBO.2001.4431_8

Characterization of the diffusion of fluorophores within microbial biofilms by fluorescence correlation microscopy under two-photon excitation

E. Guiot, M.P. Fontaine-Aupart, R. Briandet, M.N. Bellon-Fontaine, P. Georges, A. Brun, F. Merola, and B. Arrio

European Conference on Biomedical Optics 2001

Munich Germany
18–21 June 2001
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Multiphoton Microscopy (4431)

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E. Guiot, M. P. Fontaine-Aupart, R. Briandet, M. N. Bellon-Fontaine, P. Georges, A. Brun, F. Merola, and B. Arrio, "Characterization of the diffusion of fluorophores within microbial biofilms by fluorescence correlation microscopy under two-photon excitation," in Photon Migration, Optical Coherence Tomography, and Microscopy, Technical Digest Series (Optica Publishing Group, 2001), paper 4431_8.

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Abstract

The penetration and diffusion capabilities of fluorophores inside microbial biofilms of heterogeneous three-dimensional structure were studied by fluorescence correlation microscopy under two-photon excitation. The influence of the size and the surface charge of the fluorophores were investigated in order to understand the mechanisms involved in molecular diffusion inhibition occuring in biofilms such as electrostatic and steric interactions.

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