Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy

Sanjeev Kumar; Zheng Li; Chongquan Huang; Vincent Lin; Sing Yian Chew; Sing Yian Chew; Rajeev J Ram; Rajeev J Ram

doi:10.1364/COSI.2023.CM3B.6

Optica Imaging Congress (3D, COSI, DH, FLatOptics, IS, pcAOP)
Technical Digest Series (Optica Publishing Group, 2023),
paper CM3B.6
•https://doi.org/10.1364/COSI.2023.CM3B.6

Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy

Sanjeev Kumar, Zheng Li, Chongquan Huang, Vincent Lin, Sing Yian Chew, and Rajeev J Ram

Computational Optical Sensing and Imaging 2023

Boston, Massachusetts United States
14–17 August 2023
ISBN: 978-1-957171-28-9

From the session
Computational Wide Field Microscopy (CM3B)

Not Accessible

Your library or personal account may give you access

Get PDF
Email
Share
Get Citation
Copy Citation Text
S. Kumar, Z. Li, C. Huang, V. Lin, S. Y. Chew, and R. J. Ram, "Exploiting Caustics for Snapshot 3D Fluorescence Imaging in Wide-field Microscopy," in Optica Imaging Congress (3D, COSI, DH, FLatOptics, IS, pcAOP), Technical Digest Series (Optica Publishing Group, 2023), paper CM3B.6.

Export Citation
- BibTex
- Endnote (RIS)
- HTML
- Plain Text
Get Video
Citation alert
Save article

Abstract

We present a snapshot 3D fluorescence microscopy technique that extracts depth from defocus by exploiting caustics generated when spherical aberration is intentionally introduced in a high-numerical aperture microscope. The snapshot acquisition reduces the imaging time and the risk of phototoxicity associated with scanning based methods.

PDF Article | Presentation Video

More Like This

V-Shape PSF for 3D Wide Field Microscopy

Yunyang Li, Zixiao Zhang, Feng Tian, Yryx Y. Luna-Palacios, Israel Rocha-Mendoza, and Weijian Yang
ATu4Q.3 CLEO: Applications and Technology (CLEO:A&T) 2023

Double-helix microscopy for wide-field 3D single-molecule fluorescence imaging

Ginni Sharma, Sri Rama Prasanna Pavani, Sean Quirin, and Rafael Piestun
CThCC2 Conference on Lasers and Electro-Optics (CLEO:S&I) 2010

Computational imaging for 3D fluorescence microscopy

Chrysanthe Preza
IW2C.1 Imaging Systems and Applications (IS) 2014

Previous Article Next Article

Presentation Video

Presentation video access is available to:

Optica Publishing Group subscribers
Technical meeting attendees
Optica members who wish to use one of their free downloads. Please download the article first. After downloading, please refresh this page.

Contact your librarian or system administrator
or
Log in to access Optica Member Subscription or free downloads