Optimizing design of partially coherent illumination for refractive index tomographic microscopy

Jiaji Li; Jiaji Li; Jiaji Li; Ning Zhou; Ning Zhou; Ning Zhou; Zhidong Bai; Zhidong Bai; Zhidong Bai; Shun Zhou; Shun Zhou; Shun Zhou; Qian Chen; Qian Chen; Chao Zuo; Chao Zuo; Chao Zuo

doi:10.1364/COSI.2021.CM1A.6

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2021),
paper CM1A.6
•https://doi.org/10.1364/COSI.2021.CM1A.6

Optimizing design of partially coherent illumination for refractive index tomographic microscopy

Jiaji Li, Ning Zhou, Zhidong Bai, Shun Zhou, Qian Chen, and Chao Zuo

Computational Optical Sensing and Imaging 2021

Washington, DC United States
19–23 July 2021
ISBN: 978-1-943580-89-7

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Computational Microscopy I (CM1A)

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J. Li, N. Zhou, Z. Bai, S. Zhou, Q. Chen, and C. Zuo, "Optimizing design of partially coherent illumination for refractive index tomographic microscopy," in OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), H. Hua, B. Javidi, M. Martinez-Corral, O. Matoba, A. Stern, S. Thibault, T. Alieva, J. Ke, F. Willomitzer, F. Okten, P. Silveira, P. Banerjee, E. Stoykova, D. Chu, J. Park, F. Imai, C. Joo, M. Digman, D. Gardner, S. Gladysz, D. LeMaster, S. Basu, and O. Korotkova, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper CM1A.6.

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Abstract

We propose the optimizing design of illumination pattern in partially coherent optical diffraction tomography (PC-ODT), and the custom-build quantitative criterion is demonstrated to maximize the performance of POTF related to the “goodness” evaluation of an illumination aperture. Source modulation with different segment scale and gray scale is implemented to acquire arbitrary distribution source, and the corresponding three-dimensional (3D) phase optical transfer function (POTF) can be easy obtained through the numerical incoherent superstition of each segment components. Further, the simulation and experimental results is presented support this finding as well, and the proposed method is expected to find versatile applications in biological and biomedical research.

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