Comparing Individual DNA Transient Hybridization Kinetics Using DNA-PAINT and Optoplasmonic Sensing techniques

Narima Eerqing; Narima Eerqing; Subramanian Sivaraman; Subramanian Sivaraman; Jesús Rubio; Jesús Rubio; Tobias Lutz; Tobias Lutz; Hsin-Yu Wu; Hsin-Yu Wu; Janet Anders; Janet Anders; Christian Soeller; Christian Soeller; Frank Vollmer; Frank Vollmer

doi:10.1364/CLEO_QELS.2022.FW5I.2

Conference on Lasers and Electro-Optics
Technical Digest Series (Optica Publishing Group, 2022),
paper FW5I.2
•https://doi.org/10.1364/CLEO_QELS.2022.FW5I.2

Comparing Individual DNA Transient Hybridization Kinetics Using DNA-PAINT and Optoplasmonic Sensing techniques

Narima Eerqing, Subramanian Sivaraman, Jesús Rubio, Tobias Lutz, Hsin-Yu Wu, Janet Anders, Christian Soeller, and Frank Vollmer

CLEO: QELS_Fundamental Science 2022

San Jose, California United States
15–20 May 2022
ISBN: 978-1-957171-05-0

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Imaging and Sensing (FW5I)

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N. Eerqing, S. Sivaraman, J. Rubio, T. Lutz, H. Wu, J. Anders, C. Soeller, and F. Vollmer, "Comparing Individual DNA Transient Hybridization Kinetics Using DNA-PAINT and Optoplasmonic Sensing techniques," in Conference on Lasers and Electro-Optics, Technical Digest Series (Optica Publishing Group, 2022), paper FW5I.2.

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Abstract

We report a comparison of two single-molecule techniques: fluorescence nanoscopy and optoplasmonic sensing. DNA hybridization kinetics on the surface of gold nanorods are measured in both platforms, and a similar dissociation rate is demonstrated.

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