Abstract
Second Harmonic Generation (SHG) microscopy is used to image tissues rich in noncentrosymmetric proteins such as collagen [1]. Two identical noncentrosymmetric structures with opposite orientation will emit a π phase shifted SHG signal because their second order nonlinear susceptibility tensors (χ(2)) are inverted. This can be exploited to obtain additional information about the orientation of noncentrosymmetric structures in a sample by measuring the phase of the SHG signal generated in an area scanned with SHG microscopy.
© 2013 IEEE
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