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Conferences > CLEOPR > 2022 > P_CM15 > P_CM15_05

Multi-photon activation of fluorescent proteins using visible wavelength for high-resolution imaging

Toshiki Kubo, Kenta Temma, Kazunori Sugiura, Hajime Shinoda, Kai Lu, Nicholas I. Smith, Tomoki Matsuda, Takeharu Nagai, and Katsumasa Fujita

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Abstract

We experimentally demonstrated the photo-activation of reversibly photo-switchable fluorescent proteins by visible-wavelength two-photon excitation and applied the activation technique in confocal imaging of biological cells. Higher spatial resolution than conventional confocal microscopy was confirmed.

© 2022 IEEE

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