Abstract
Summary: Recording of time-resolved FRET images was achieved by combining a confocal laser scanning microscope (Zeiss LSM-510 Axiovert), a 150 femtosecond Coherent Titanium Sapphire Laser and an advanced Time-Correlated Single Photon Counting (TCSPC) imaging technique developed by Becker & Hickl, Berlin. The principle of the SPC-730 TCSPC Imaging module is shown in fig. 1.
© 2001 Optical Society of America
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