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Conferences > CLEO > 2000 > CFF > CFF2
  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 2000),
  • paper CFF2

Three-dimensional orientation determination of single molecules by emission pattern characterization

A. P. Bartko and R. M. Dickson

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Abstract

We have employed wide-field total internal reflection (TIR) through a high numerical aperture microscope objective to simultaneously excite many individual x, y, and z-oriented fluorescent DilC181 molecules. In an ideal imaging system, all fluorophores appear as identical, diffraction-limited Airy patterns.2

© 2000 Optical Society of America

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