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Conferences > BRAIN > 2023 > BTu1B > BTu1B.6

Development of 3-Photon Functional Imaging in Deep Layers of Macaque Cortex

Kevin T. Takasaki, Gaku Hatanaka, Soumya Chatterjee, Pooja Balaram, Taekjun Kim, Celeste Dylla, Farzaneh Abolmaali, R. Clay Reid, Anitha Pasupathy, Wyeth Bair, and Jack Waters

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Abstract

3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.

© 2023 The Author(s)

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