Reference frame free correction of retinal motion artifacts present during in vivo retinal imaging. Yes, it is now possible with the application of a dual raster scanning imaging system, initially developed by the Stephen A. Burns Laboratory at Indiana University Bloomington, USA, to study red blood cell motion in retinal capillaries with an adaptive optics scanning laser ophthalmoscope (AO-SLO). In the latest paper from this group, Ting Luo et al. showcase a great advantage offered by their system and produces reference frame free, i.e. without prior knowledge of the undistorted image, motion-corrected retinal images. In the dual scanning imaging system, the target (retina) is imaged simultaneously with two imaging beams that share a common pupil but are spatially separated on the imaging plane. This spatial shift between two raster scanning beams results in the acquisition of an identical region twice, with a well-defined time delay that can be adjusted by changing spatial separation between beams. The spatial and temporal relations between two images allow an accurate measure of target (retinal) motion providing motion correction information. Additionally, because the current method uses data acquired within the frame, it may be possible to implement real-time image stabilization with rapid recovery following blinks and saccades.
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