Three-dimensional time lapse anisotropy images of EGFP-paxillin expressing CHO-K1 cells. As the cells shown in the 3D intensity projections travel along the surface, membrane protrusions are formed (arrows). At these sites, EGFP-paxillin is compacted forming new adhesions as indicated by the low anisotropy. For details, see Hedde et al., Opt. Express 23, 22308-22317 (2015).