In the current study by Lee et al., the authors use hyperspectral imaging to determine hemoglobin oxygenation status from the endogenous absorption spectra of blood within the microvessels. This technique is combined with administration of exogenous near infrared (NIR) fluorescent liposomes, termed first pass fluorescence (FPF) imaging, to detect the entrance of the NIR fluorophores into the microcirculation. FPF imaging provides information about blood flow, microvessel morphology, and microvessel network connections through measurement of the blood supply time within the field of view. These imaging modalities were used together to study the microvasculature development of human renal cell carcinoma tumors implanted in mouse dorsal skinfold window chamber models. Microvasculature development was monitored over a four-day time period following tumor implantation in the window chamber. Using both hyperspectal imaging and FPF imaging both the increased speed of blood flow and oxygenation status of the tumor tissue could be seen by the fourth day of imaging. This combined imaging technique also demonstrated insight into how vascular malformations such as ateriovenous shunts create an oxygenated environment in which an immature tumor can proliferate. Since this technique enables serial imaging over multiple days it may be useful for studying therapeutic methods to normalize diseased tissue vasculature in the future.
Thalangunam Krishnaswamy S.
05/12/2013 3:12 AM
Any studies related to a malignant tumorous growth resulting from blood vessels of diseased tissue combining both NIR and FPF imaging?.How does it vary in the case of a benign tumorous cell study?
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