Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Singlet oxygen phosphorescence imaging by superconducting single-photon detector and time-correlated single-photon counting

Not Accessible

Your library or personal account may give you access

Abstract

This Letter presents, to the best of our knowledge, a novel optical configuration for direct time-resolved measurements of luminescence from singlet oxygen, both in solutions and from cultured cells on photodynamic therapy. The system is based on the superconducting single-photon detector, coupled to the confocal scanner that is modified for the near-infrared measurements. The recording of a phosphorescence signal from singlet oxygen at 1270 nm has been done using time-correlated single-photon counting. The performance of the system is verified by measuring phosphorescence from singlet oxygen generated by the photosensitizers commonly used in photodynamic therapy: methylene blue and chlorin e6. The described system can be easily upgraded to the configuration when both phosphorescence from singlet oxygen and fluorescence from the cells can be detected in the imaging mode. Thus, co-localization of the signal from singlet oxygen with the areas inside the cells can be done.

© 2021 Optical Society of America

Full Article  |  PDF Article

Corrections

Pavel Morozov, Maria Lukina, Marina Shirmanova, Alexander Divochiy, Varvara Dudenkova, Gregory N. Gol’tsman, Wolfgang Becker, and Vladislav I. Shcheslavskiy, "Singlet oxygen phosphorescence imaging by superconducting single-photon detector and time-correlated single-photon counting: publisher’s note," Opt. Lett. 46, 1582-1582 (2021)
https://opg.optica.org/ol/abstract.cfm?uri=ol-46-7-1582

More Like This
Singlet oxygen phosphorescence imaging by superconducting single-photon detector and time-correlated single-photon counting: publisher’s note

Pavel Morozov, Maria Lukina, Marina Shirmanova, Alexander Divochiy, Varvara Dudenkova, Gregory N. Gol’tsman, Wolfgang Becker, and Vladislav I. Shcheslavskiy
Opt. Lett. 46(7) 1582-1582 (2021)

Singlet oxygen luminescence detection with a fiber-coupled superconducting nanowire single-photon detector

Nathan R. Gemmell, Aongus McCarthy, Baochang Liu, Michael G. Tanner, Sander D. Dorenbos, Valery Zwiller, Michael S. Patterson, Gerald S. Buller, Brian C. Wilson, and Robert H. Hadfield
Opt. Express 21(4) 5005-5013 (2013)

Time-resolved singlet-oxygen luminescence detection with an efficient and practical semiconductor single-photon detector

Gianluca Boso, Damei Ke, Boris Korzh, Jordan Bouilloux, Norbert Lange, and Hugo Zbinden
Biomed. Opt. Express 7(1) 211-224 (2016)

Supplementary Material (1)

NameDescription
Supplement 1       Performance of the SSPD. System detection efficiency vs bias current (blue dots). Dark count rate vs bias current (black dots).

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved