Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Two-photon microscopy with a frequency-doubled fully fusion-spliced fiber laser at 1840 nm

Not Accessible

Your library or personal account may give you access

Abstract

We introduce a fiber-based laser system providing 130 fs pulses with 3.5 nJ energy at 920 nm at a 43 MHz repetition rate and illustrate the potential of the source for two-photon excited fluorescence microscopy of living mouse brain. The laser source is based on frequency-doubling high-energy solitons generated and frequency-shifted to 1840 nm in large mode area fibers. This simple laser system could unleash the potential of two-photon microscopy techniques in the biology laboratory where green fluorescent proteins with two-photon absorption spectrum peaking around 920 nm are routinely used.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
All-fiberized 1840-nm femtosecond thulium fiber laser for label-free nonlinear microscopy

Duanyang Xu, Konstantinos N. Bourdakos, Anna Crisford, Peter Johnson, Ibrahim Abughazaleh, Panuwat Srisamran, Richard O. C. Oreffo, Sumeet Mahajan, David J. Richardson, and Lin Xu
Biomed. Opt. Express 14(9) 4520-4530 (2023)

890-nm-excited SHG and fluorescence imaging enabled by an all-fiber mode-locked laser

Hongsen He, Meng Zhou, Tian Qiao, Hei Ming Lai, Qiao Ran, Yu-Xuan Ren, Ho Ko, Chaogu Zheng, Kevin K. Tsia, and Kenneth K. Y. Wong
Opt. Lett. 47(11) 2710-2713 (2022)

Generation of 35  kW peak power 80  fs pulses at 2.9  μm from a fully fusion-spliced fiber laser

H. Delahaye, G. Granger, J.-T. Gomes, L. Lavoute, D. Gaponov, N. Ducros, and S. Fevrier
Opt. Lett. 44(9) 2318-2321 (2019)

Supplementary Material (3)

NameDescription
Visualization 1       Animated 240 μm thick volume stack (60 slices, 4 μm z-step) showing deep imaging on an eGFP mouse brain.
Visualization 2       Animated 200 μm thick z-stack (51 slices, 4 μm z-step) showing deep imaging in the subcutaneous tissue of an e-GFP mouse head.
Visualization 3       Animated 200 μm thick z-stack (51 slices, 4 μm z-step) showing imaging through the right parietal bone of an e-GFP mouse head.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved