Abstract
We demonstrate that the spatial resolution in confocal laser scanning microscopy is remarkably improved by simple image subtraction between images acquired by higher-order transverse modes of a cylindrical vector beam, referred to as radially and azimuthally polarized Laguerre–Gaussian mode beams. Two types of combinations of vector mode beams suitable for subtraction imaging are derived by a systematical study based on numerical calculations. The spatial resolution of about 100 nm is experimentally achieved without the degradation of image quality.
© 2019 Optical Society of America
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