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Millisecond two-photon optical ribbon imaging for small-animal functional connectome study

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Abstract

We developed a high-speed two-photon optical ribbon imaging system, which combines galvo-mirrors for an arbitrary curve scan on a lateral plane and a tunable acoustic gradient-index lens for a 100 kHz–1 MHz axial scan. The system provides micrometer/millisecond spatiotemporal resolutions, which enable continuous readout of functional dynamics from small and densely packed neurons in a living adult Drosophila brain. Compared to sparse sampling techniques, the ribbon imaging modality avoids motion artifacts. Combined with a Drosophila anatomical connectome database, which is the most complete among all model animals, this technique paves the way toward establishing whole-brain functional connectome.

© 2019 Optical Society of America

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Supplementary Material (1)

NameDescription
Visualization 1       Ribbon-scan images of mushroom body in a Drosophila brain, corresponding to Fig. 3b in the main text. It shows that the whole MB structures stay stationary without noticeable movements throughout the stimulation.

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