Abstract
A dual-wavelength digital holographic phase and fluorescence microscopy system is demonstrated as the decoding and detection platform of an optical thickness encoded suspension array. The phase imaging path is designed to decode optical thickness encoded microcarriers, and the fluorescence imaging path is used to detect the quantitative information of the bound target analytes. The encoding capacity could be more than 100. The decoding reliability of the phase imaging path is verified by a multiplexed immunoassay experiment. The ability for quantitative analysis of the fluorescence imaging path is confirmed by concentration gradient experiments. This method offers high decoding accuracy and high detection sensitivity of label signals.
© 2018 Optical Society of America
Full Article | PDF ArticleMore Like This
Xuejing Chen, Zhiyuan Shen, Yonghong He, Tian Guan, Qinghua He, Bei Wang, Luyuan Xie, Guangxia Feng, Bangrong Lu, Xuesi Zhou, Jiafan Liu, and Zhigang Fan
Opt. Express 27(3) 1894-1910 (2019)
Rongli Guo, Weiguang Zhang, Rong Liu, Cunli Duan, and Fan Wang
Opt. Lett. 43(14) 3449-3452 (2018)
Junwei Min, Meiling Zhou, Xun Yuan, Kai Wen, Xianghua Yu, Tong Peng, and Baoli Yao
Opt. Lett. 43(18) 4469-4472 (2018)