Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Tomographic phosphorescence lifetime multiplexing

Not Accessible

Your library or personal account may give you access

Abstract

We present a tomographic reconstruction algorithm for recovering distributions of multiple phosphorescent dyes within turbid media from time-resolved measurements, using either point or spatially patterned sources and detectors. The algorithm employs a multi-exponential analysis of time-resolved data, followed by tomographic inversion of the decay amplitudes to recover independent yield distributions for each lifetime present in the medium. Using Monte Carlo simulations, we computationally demonstrate that this two-step inversion approach provides several-fold improvement in quantitative and localization accuracy compared to a direct inversion of the time domain phosphorescence. We also demonstrate the tomographic reconstruction of up to three phosphorescent lifetimes embedded in thick tissue. The proposed algorithm can allow quantitative multiplexed tomography of luminescent and phosphorescent dyes for a wide range of in vivo applications.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
Tomographic fluorescence lifetime multiplexing in the spatial frequency domain

Anand T. N. Kumar, Steven S. Hou, and William L. Rice
Optica 5(5) 624-627 (2018)

Optimal estimator for tomographic fluorescence lifetime multiplexing

Steven S. Hou, Brian J. Bacskai, and Anand T. N. Kumar
Opt. Lett. 41(7) 1352-1355 (2016)

Comparison of tomographic fluorescence spectral and lifetime multiplexing

Steven S. Hou, Brian J. Bacskai, and Anand T. N. Kumar
Opt. Lett. 41(22) 5337-5340 (2016)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (11)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved