Abstract

The method of measuring blood flow in photoacoustic microscopy usually relies on ultrasonic transducers in contact fashion, which is not favored in many applications, such as wound areas, burns, and anabrosis. Here we present a noncontact photoacoustic velocity measurement method to quantitatively map transverse blood flow based on the photoacoustic Doppler (PAD) bandwidth broadening method with an all-optical photoacoustic microscopy system. It is validated that the PAD bandwidth broadening is proportional to the transverse flow within a certain range. The transverse flow speed ranging from 0 to 5.5 mm/s, as well as sectional flow images, was obtained in the blood-mimicking flow phantoms. Furthermore, the blood flow image of the mouse ear demonstrates that the all-optical photoacoustic Doppler method can acquire the information of blood flow in vivo, which could significantly broaden the scope of applications for obtaining the blood flow velocity of the microvasculature in biomedicine.

© 2018 Optical Society of America

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