Abstract

We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method.

© 2017 Optical Society of America

Full Article  |  PDF Article

Corrections

10 January 2017: A correction was made to the author listing.


OSA Recommended Articles
Off-axis tilt compensation in common-path digital holographic microscopy based on hologram rotation

Dingnan Deng, Weijuan Qu, Wenqi He, Yu Wu, Xiaoli Liu, and Xiang Peng
Opt. Lett. 42(24) 5282-5285 (2017)

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Pierre Marquet, Benjamin Rappaz, Pierre J. Magistretti, Etienne Cuche, Yves Emery, Tristan Colomb, and Christian Depeursinge
Opt. Lett. 30(5) 468-470 (2005)

Simple and flexible phase compensation for digital holographic microscopy with electrically tunable lens

DingNan Deng, JunZheng Peng, WeiJuan Qu, Yu Wu, XiaoLi Liu, WenQi He, and Xiang Peng
Appl. Opt. 56(21) 6007-6014 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (1)

NameDescription
» Visualization 1: AVI (9882 KB)      Fast motion movie of living fibroblast cells.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription