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Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy

Junwei Min, Baoli Yao, Steffi Ketelhut, Christian Engwer, Burkhard Greve, and Björn Kemper

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Abstract

We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method.

© 2017 Optical Society of America

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Corrections

10 January 2017: A correction was made to the author listing.

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Supplementary Material (1)

NameDescription
Visualization 1: AVI (9882 KB)      Fast motion movie of living fibroblast cells.

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