Abstract

We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.

© 2017 Optical Society of America

Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber

Benjamin E. Sherlock, Cai Li, Xiangnan Zhou, Alba Alfonso-Garcia, Julien Bec, Diego Yankelevich, and Laura Marcu
Opt. Lett. 44(9) 2302-2305 (2019)

Dual modality intravascular catheter system combining pulse-sampling fluorescence lifetime imaging and polarization-sensitive optical coherence tomography

Julien Bec, Xiangnan Zhou, Martin Villiger, Jeffrey A. Southard, Brett Bouma, and Laura Marcu
Biomed. Opt. Express 15(4) 2114-2132 (2024)

Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography

Hyeong Soo Nam, Woo Jae Kang, Min Woo Lee, Joon Woo Song, Jin Won Kim, Wang-Yuhl Oh, and Hongki Yoo
Biomed. Opt. Express 9(4) 1930-1947 (2018)

A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization

Jesung Park, Javier A. Jo, Sebina Shrestha, Paritosh Pande, Qiujie Wan, and Brian E. Applegate
Biomed. Opt. Express 1(1) 186-200 (2010)

Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence

Sebina Shrestha, Michael J. Serafino, Jesus Rico-Jimenez, Jesung Park, Xi Chen, Siqin Zhaorigetu, Brian L. Walton, Javier A. Jo, and Brian E. Applegate
Biomed. Opt. Express 7(9) 3184-3197 (2016)