Abstract

We have developed a trimodality imaging system by optically integrating all-optical photoacoustic microscopy (AOPAM), optical coherence tomography (OCT), and fluorescence microscopy (FLM) to provide complementary information including optical absorption, optical back-scattering, and fluorescence contrast of biological tissue. By sharing the same low-coherence Michelson interferometer, AOPAM and OCT could be organically optically combined. Also, owing to using the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence signals are obtained in a single pulse. Simultaneously photoacoustic angiography, tissue structure, and fluorescence molecular in vivo images of mouse ear were acquired to demonstrate the capabilities of the optically integrated trimodality imaging system, which can present more information to study tumor angiogenesis, vasculature, anatomical structure, and microenvironments in vivo.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography

Shuliang Jiao, Zhixing Xie, Hao F. Zhang, and Carmen A. Puliafito
Opt. Lett. 34(19) 2961-2963 (2009)

All-optically integrated photo-acoustic microscopy and optical coherence tomography based on a single Michelson detector

Zhongjiang Chen, Sihua Yang, Yi Wang, and Da Xing
Opt. Lett. 40(12) 2838-2841 (2015)

Trimodality imaging system and intravascular endoscopic probe: combined optical coherence tomography, fluorescence imaging and ultrasound imaging

Shanshan Liang, Teng Ma, Joseph Jing, Xiang Li, Jiawen Li, K. Kirk Shung, Qifa Zhou, Jun Zhang, and Zhongping Chen
Opt. Lett. 39(23) 6652-6655 (2014)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription