Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Beam scanning for rapid coherent Raman hyperspectral imaging

Not Accessible

Your library or personal account may give you access

Abstract

Coherent Raman imaging requires high-peak power laser pulses to maximize the nonlinear multiphoton signal generation, but accompanying photo-induced sample damage often poses a challenge to microscopic imaging studies. We demonstrate that beam scanning by a 3.5-kHz resonant mirror in a broadband coherent anti-Stokes Raman scattering (BCARS) imaging system can reduce photo-induced damage without compromising signal intensity. Additionally, beam scanning enables slit acquisition, in which spectra from a thin line of sample illumination are acquired in parallel during a single charge-coupled device exposure. Reflective mirrors are employed in the beam-scanning assembly to minimize chromatic aberration and temporal dispersion. The combined approach of beam scanning and slit acquisition is compared with the sample-scanning mode in terms of spatial resolution, photo-induced damage, and imaging speed at the maximum laser power below the sample-damage threshold. We show that the beam-scanning BCARS imaging method can reduce photodamage probability in biological cells and tissues, enabling faster imaging speed by using a higher excitation laser power than could be achieved without beam scanning.

Full Article  |  PDF Article
More Like This
Rapid coherent Raman hyperspectral imaging based on delay-spectral focusing dual-comb method and deep learning algorithm

Yujia Zhang, Minjian Lu, Jiaqi Hu, Yan Li, Perry Ping Shum, Jinna Chen, and Haoyun Wei
Opt. Lett. 48(3) 550-553 (2023)

High-speed stimulated hyperspectral Raman imaging using rapid acousto-optic delay lines

Mohammed S. Alshaykh, Chien-Sheng Liao, Oscar E. Sandoval, Gregory Gitzinger, Nicolas Forget, Daniel E. Leaird, Ji-Xin Cheng, and Andrew M. Weiner
Opt. Lett. 42(8) 1548-1551 (2017)

Rapid scanning catheterscope for expanded forward-view volumetric imaging with optical coherence tomography

Kristen L. Lurie, Abhijit A. Gurjarpadhye, Eric J. Seibel, and Audrey K. Ellerbee
Opt. Lett. 40(13) 3165-3168 (2015)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved