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Two-photon microscopy by wavelength-swept pulses delivered through single-mode fiber

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Abstract

Nonlinear microscopy through flexible fiber-optic catheters has potential in clinical diagnostic applications. Here, we demonstrate a new approach based on wavelength-swept narrowband pulses that permits simple fiber-optic delivery without need of the dispersion management and allows nonmechanical beam scanning. Using 0.86ps pulses rapidly tuned from 789nmto822nm at a sweep rate of 200Hz, we demonstrate two-photon fluorescence and second-harmonic generation imaging through a 5-m-long standard single-mode fiber.

© 2010 Optical Society of America

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