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High-speed wave-mixing laser Doppler imaging in vivo

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Abstract

An interferometric method for parallel optical spectroscopy in the kilohertz range is reported, as well as its experimental validation in the context of high-speed laser Doppler imaging in vivo. The interferometric approach enables imaging in the low light conditions of a 2kHz frame rate recording with a complementary metal-oxide semiconductor camera. Observation of mice craniums with near-infrared (λ=785nm) laser light in reflection configuration is reported. Doppler spectral images allegedly sensitive to blood flow are sequentially measured at several optical frequency detunings, to shift the spectral range of analysis in the radio-frequency spectrum.

© 2008 Optical Society of America

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