Abstract

We have developed a wide-field total-internal-reflection fluorescence microscope capable of imaging single molecules in live cells, resolved in both wavelength and polarization. We show fluorescence resonance energy transfer between single pairs of fluorescent molecules bound to signaling receptors in the plasma membrane of live cells and demonstrate the importance of polarization discrimination in addition to wavelength separation.

© 2006 Optical Society of America

Simultaneous widefield single molecule orientation and FRET microscopy in cells

S. E. D. Webb, D. J. Rolfe, S. R. Needham, S. K. Roberts, D. T. Clarke, C. I. McLachlan, M. P. Hobson, and M. L. Martin-Fernandez
Opt. Express 16(25) 20258-20265 (2008)

Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy

Ruei-Yu He, Guan-Liang Chang, Hua-Lin Wu, Chi-Hung Lin, Kuo-Chih Chiu, Yuan-Deng Su, and Shean-Jen Chen
Opt. Express 14(20) 9307-9316 (2006)

Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy

Euiheon Chung, Daekeun Kim, and Peter T. So
Opt. Lett. 31(7) 945-947 (2006)

Thin-film-based sensitivity enhancement for total internal reflection fluorescence live-cell imaging

Kyujung Kim, Eun-Jin Cho, Yong-Min Huh, and Donghyun Kim
Opt. Lett. 32(21) 3062-3064 (2007)

Multicolour single molecule imaging on cells using a supercontinuum source

Stephen E. D. Webb, Laura Zanetti-Domingues, Benjamin C. Coles, Daniel J. Rolfe, Richard J. Wareham, and Marisa L. Martin-Fernandez
Biomed. Opt. Express 3(3) 400-406 (2012)