Abstract
The thermal disruption of collagen I in rat tail tendon is investigated with second-harmonic generation (SHG) microscopy. We investigate its effects on SHG images and intensity in the temperature range . We find that the SHG signal decreases rapidly starting at . However, SHG imaging reveals that breakage of collagen fibers is not evident until and worsens with increasing temperature. At , structures of both molten and fibrous collagen exist, and the disruption of collagen appears to be complete at . Our results suggest that, in addition to intensity measurement, SHG imaging is necessary for monitoring details of thermally induced changes in collagen structures in biomedical applications.
© 2005 Optical Society of America
Full Article | PDF ArticleMore Like This
Sung-Jan Lin, Ruei-Jr Wu, Hsin-Yuan Tan, Wen Lo, Wei-Chou Lin, Tai-Horng Young, Chih-Jung Hsu, Jau-Shiuh Chen, Shiou-Hwa Jee, and Chen-Yuan Dong
Opt. Lett. 30(17) 2275-2277 (2005)
Raghu Ambekar Ramachandra Rao, Monal R. Mehta, Scott Leithem, and Kimani C. Toussaint, Jr.
Opt. Lett. 34(24) 3779-3781 (2009)
Jingying Zhang, Junting Chen, Yang Wang, and Rui Li
Appl. Opt. 60(30) 9295-9302 (2021)