Abstract

In 4Pi fluorescence microscopy the point-spread function is composed of a strong central lobe accompanied by interference sidelobes that produce artifacts in the image. We propose to combine two-color two-photon fluorescence microscopy and 4Pi fluorescence microscopy to overcome this sidelobe problem. Simulation results show that a single sharp fluorescence spot can be produced by use of two excitation wavelengths of 400 and 800 nm and detected at 350-nm emission wavelength.

© 2004 Optical Society of America

4Pi microscopy of type A with 1-photon excitation in biological fluorescence imaging

Marion Lang, Tobias Müller, Johann Engelhardt, and Stefan W. Hell
Opt. Express 15(5) 2459-2467 (2007)

4Pi confocal microscopy with alternate interference

Stefan W. Hell and Matthias Nagorni
Opt. Lett. 23(20) 1567-1569 (1998)

Polarization effects in 4Pi confocal microscopy studied with water-immersion lenses

Karsten Bahlmann and Stefan W. Hell
Appl. Opt. 39(10) 1652-1658 (2000)

Multiple-objective microscopy with three-dimensional resolution near 100 nm and a long working distance

O. Haeberlé, C. Xu, A. Dieterlen, and S. Jacquey
Opt. Lett. 26(21) 1684-1686 (2001)

Axial apodization in 4Pi-confocal microscopy by annular binary filters

Manuel Martı́nez-Corral, Amparo Pons, and Marı́a-Teresa Caballero
J. Opt. Soc. Am. A 19(8) 1532-1536 (2002)