Abstract

In this paper, we present a modified SOFI algorithm with enhanced temporal resolution: the required number of raw images for SOFI is reduced from hundreds to tens. The modification is intended to eliminate the low-frequency fluctuation and readout noise from the raw image stack, and is achieved by separately utilizing two wavelet-based filters in the temporal and spatial domains of the raw image stack. The high-frequency stochastic fluctuating signal could be extracted effectively, and the efficiency of SOFI could be enhanced. The modified SOFI image could be generated with 25 frames of raw images, and the corresponding acquisition time was 1.25 s.

© 2016 Optical Society of America

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References

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2015 (6)

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

S. C. Stein, A. Huss, D. Hähnel, I. Gregor, and J. Enderlein, “Fourier interpolation stochastic optical fluctuation imaging,” Opt. Express 23(12), 16154–16163 (2015).
[Crossref] [PubMed]

X. Chen, Z. Zeng, H. Wang, and X. Peng, “Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes,” Nano Res. 8(7), 2251–2260 (2015).
[Crossref]

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

2014 (2)

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

2012 (2)

T. Dertinger, J. Xu, O. F. Naini, R. Vogel, and S. Weiss, “SOFI-based 3D superresolution sectioning with a widefield microscope,” Opt. Nanoscopy 1(1), 2 (2012).
[Crossref] [PubMed]

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

2011 (1)

2010 (3)

T. Dertinger, M. Heilemann, R. Vogel, M. Sauer, and S. Weiss, “Superresolution optical fluctuation imaging with organic dyes,” Angew. Chem. Int. Ed. Engl. 49(49), 9441–9443 (2010).
[Crossref] [PubMed]

T. M. Watanabe, S. Fukui, T. Jin, F. Fujii, and T. Yanagida, “Real-time nanoscopy by using blinking enhanced quantum dots,” Biophys. J. 99(7), L50–L52 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, R. Vogel, J. Enderlein, and S. Weiss, “Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI),” Opt. Express 18(18), 18875–18885 (2010).
[Crossref] [PubMed]

2009 (2)

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

2006 (2)

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[Crossref] [PubMed]

1999 (1)

1994 (1)

Baird, M. A.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Bates, M.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[Crossref] [PubMed]

Beach, J. R.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Berclaz, C.

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

Betzig, E.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Bewersdorf, J.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Bocchio, N. L.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

Bonifacino, J. S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Booth, M. J.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Castello, M.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Chen, B. C.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Chen, X.

X. Chen, Z. Zeng, H. Wang, and X. Peng, “Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes,” Nano Res. 8(7), 2251–2260 (2015).
[Crossref]

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

Chhun, B. B.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Cognet, L.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Colyer, R.

T. Dertinger, R. Colyer, R. Vogel, J. Enderlein, and S. Weiss, “Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI),” Opt. Express 18(18), 18875–18885 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Cordes, T.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Davidson, M. W.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Davis, S. J.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Dellagiacoma, C.

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

S. Geissbuehler, C. Dellagiacoma, and T. Lasser, “Comparison between SOFI and STORM,” Biomed. Opt. Express 2(3), 408–420 (2011).
[Crossref] [PubMed]

Dertinger, T.

T. Dertinger, J. Xu, O. F. Naini, R. Vogel, and S. Weiss, “SOFI-based 3D superresolution sectioning with a widefield microscope,” Opt. Nanoscopy 1(1), 2 (2012).
[Crossref] [PubMed]

T. Dertinger, M. Heilemann, R. Vogel, M. Sauer, and S. Weiss, “Superresolution optical fluctuation imaging with organic dyes,” Angew. Chem. Int. Ed. Engl. 49(49), 9441–9443 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, R. Vogel, J. Enderlein, and S. Weiss, “Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI),” Opt. Express 18(18), 18875–18885 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Diaspro, A.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Dubikovskaya, E. A.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Eggeling, C.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Enderlein, J.

S. C. Stein, A. Huss, D. Hähnel, I. Gregor, and J. Enderlein, “Fourier interpolation stochastic optical fluctuation imaging,” Opt. Express 23(12), 16154–16163 (2015).
[Crossref] [PubMed]

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, R. Vogel, J. Enderlein, and S. Weiss, “Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI),” Opt. Express 18(18), 18875–18885 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Ewers, H.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Fujii, F.

T. M. Watanabe, S. Fukui, T. Jin, F. Fujii, and T. Yanagida, “Real-time nanoscopy by using blinking enhanced quantum dots,” Biophys. J. 99(7), L50–L52 (2010).
[Crossref] [PubMed]

Fukui, S.

T. M. Watanabe, S. Fukui, T. Jin, F. Fujii, and T. Yanagida, “Real-time nanoscopy by using blinking enhanced quantum dots,” Biophys. J. 99(7), L50–L52 (2010).
[Crossref] [PubMed]

Geissbuehler, S.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

S. Geissbuehler, C. Dellagiacoma, and T. Lasser, “Comparison between SOFI and STORM,” Biomed. Opt. Express 2(3), 408–420 (2011).
[Crossref] [PubMed]

Gisou van der Goot, F.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Godinat, A.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Gregor, C.

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

Gregor, I.

Griffis, E. R.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Gustafsson, M. G.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Hähnel, D.

Hammer, J. A.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Heilemann, M.

T. Dertinger, M. Heilemann, R. Vogel, M. Sauer, and S. Weiss, “Superresolution optical fluctuation imaging with organic dyes,” Angew. Chem. Int. Ed. Engl. 49(49), 9441–9443 (2010).
[Crossref] [PubMed]

Heintzmann, R.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Hell, S. W.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

T. A. Klar and S. W. Hell, “Subdiffraction resolution in far-field fluorescence microscopy,” Opt. Lett. 24(14), 954–956 (1999).
[Crossref] [PubMed]

S. W. Hell and J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy,” Opt. Lett. 19(11), 780–782 (1994).
[Crossref] [PubMed]

Herholt, A.

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

Hess, H.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Hess, H. F.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Honigmann, A.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Huang, N.

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

Huss, A.

S. C. Stein, A. Huss, D. Hähnel, I. Gregor, and J. Enderlein, “Fourier interpolation stochastic optical fluctuation imaging,” Opt. Express 23(12), 16154–16163 (2015).
[Crossref] [PubMed]

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Iyer, G.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Jakobs, S.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Jensen, N. A.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Jin, T.

T. M. Watanabe, S. Fukui, T. Jin, F. Fujii, and T. Yanagida, “Real-time nanoscopy by using blinking enhanced quantum dots,” Biophys. J. 99(7), L50–L52 (2010).
[Crossref] [PubMed]

Kirchhausen, T.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Klar, T. A.

Klenerman, D.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Kner, P.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Lasser, T.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

S. Geissbuehler, C. Dellagiacoma, and T. Lasser, “Comparison between SOFI and STORM,” Biomed. Opt. Express 2(3), 408–420 (2011).
[Crossref] [PubMed]

Leutenegger, M.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

Li, D.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Lindwasser, O. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Lippincott-Schwartz, J.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Lounis, B.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Milkie, D. E.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Moses, B.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Naini, O. F.

T. Dertinger, J. Xu, O. F. Naini, R. Vogel, and S. Weiss, “SOFI-based 3D superresolution sectioning with a widefield microscope,” Opt. Nanoscopy 1(1), 2 (2012).
[Crossref] [PubMed]

Olenych, S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Pasham, M.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Patterson, G. H.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Peng, J.

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

Peng, X.

X. Chen, Z. Zeng, H. Wang, and X. Peng, “Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes,” Nano Res. 8(7), 2251–2260 (2015).
[Crossref]

Rossner, M. J.

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

Rust, M. J.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[Crossref] [PubMed]

Sahl, S. J.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Sandoz, P. A.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Sauer, M.

T. Dertinger, M. Heilemann, R. Vogel, M. Sauer, and S. Weiss, “Superresolution optical fluctuation imaging with organic dyes,” Angew. Chem. Int. Ed. Engl. 49(49), 9441–9443 (2010).
[Crossref] [PubMed]

Shan, C.

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

Shao, L.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Sharipov, A.

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Shtengel, G.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Sougrat, R.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Steffens, H.

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

Stein, S. C.

Sun, Y.

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

Teng, J.

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

Testa, I.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Tinnefeld, P.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Vicidomini, G.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Vogel, R.

T. Dertinger, J. Xu, O. F. Naini, R. Vogel, and S. Weiss, “SOFI-based 3D superresolution sectioning with a widefield microscope,” Opt. Nanoscopy 1(1), 2 (2012).
[Crossref] [PubMed]

T. Dertinger, M. Heilemann, R. Vogel, M. Sauer, and S. Weiss, “Superresolution optical fluctuation imaging with organic dyes,” Angew. Chem. Int. Ed. Engl. 49(49), 9441–9443 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, R. Vogel, J. Enderlein, and S. Weiss, “Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI),” Opt. Express 18(18), 18875–18885 (2010).
[Crossref] [PubMed]

Wang, H.

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

X. Chen, Z. Zeng, H. Wang, and X. Peng, “Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes,” Nano Res. 8(7), 2251–2260 (2015).
[Crossref]

Watanabe, T. M.

T. M. Watanabe, S. Fukui, T. Jin, F. Fujii, and T. Yanagida, “Real-time nanoscopy by using blinking enhanced quantum dots,” Biophys. J. 99(7), L50–L52 (2010).
[Crossref] [PubMed]

Weiss, S.

T. Dertinger, J. Xu, O. F. Naini, R. Vogel, and S. Weiss, “SOFI-based 3D superresolution sectioning with a widefield microscope,” Opt. Nanoscopy 1(1), 2 (2012).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, R. Vogel, J. Enderlein, and S. Weiss, “Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI),” Opt. Express 18(18), 18875–18885 (2010).
[Crossref] [PubMed]

T. Dertinger, M. Heilemann, R. Vogel, M. Sauer, and S. Weiss, “Superresolution optical fluctuation imaging with organic dyes,” Angew. Chem. Int. Ed. Engl. 49(49), 9441–9443 (2010).
[Crossref] [PubMed]

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Wichmann, J.

Willig, K. I.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

Winoto, L.

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Xi, P.

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

Xu, J.

T. Dertinger, J. Xu, O. F. Naini, R. Vogel, and S. Weiss, “SOFI-based 3D superresolution sectioning with a widefield microscope,” Opt. Nanoscopy 1(1), 2 (2012).
[Crossref] [PubMed]

Xu, P.

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Yanagida, T.

T. M. Watanabe, S. Fukui, T. Jin, F. Fujii, and T. Yanagida, “Real-time nanoscopy by using blinking enhanced quantum dots,” Biophys. J. 99(7), L50–L52 (2010).
[Crossref] [PubMed]

Zeng, Z.

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

X. Chen, Z. Zeng, H. Wang, and X. Peng, “Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes,” Nano Res. 8(7), 2251–2260 (2015).
[Crossref]

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

Zhang, H.

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

Zhang, M.

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

Zhang, X.

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

Zhuang, X.

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[Crossref] [PubMed]

ACS Nano (1)

X. Zhang, X. Chen, Z. Zeng, M. Zhang, Y. Sun, P. Xi, J. Peng, and P. Xu, “Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI),” ACS Nano 9(3), 2659–2667 (2015).
[Crossref] [PubMed]

Angew. Chem. Int. Ed. Engl. (1)

T. Dertinger, M. Heilemann, R. Vogel, M. Sauer, and S. Weiss, “Superresolution optical fluctuation imaging with organic dyes,” Angew. Chem. Int. Ed. Engl. 49(49), 9441–9443 (2010).
[Crossref] [PubMed]

Biomed. Opt. Express (1)

Biophys. J. (2)

T. M. Watanabe, S. Fukui, T. Jin, F. Fujii, and T. Yanagida, “Real-time nanoscopy by using blinking enhanced quantum dots,” Biophys. J. 99(7), L50–L52 (2010).
[Crossref] [PubMed]

K. I. Willig, H. Steffens, C. Gregor, A. Herholt, M. J. Rossner, and S. W. Hell, “Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse,” Biophys. J. 106(1), L01–L03 (2014).
[Crossref] [PubMed]

J. Phys. D Appl. Phys. (1)

S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J. Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, L. Cognet, B. Lounis, H. Ewers, S. J. Davis, C. Eggeling, D. Klenerman, K. I. Willig, G. Vicidomini, M. Castello, A. Diaspro, and T. Cordes, “The 2015 super-resolution microscopy roadmap,” J. Phys. D Appl. Phys. 48(44), 443001 (2015).
[Crossref]

Nano Res. (1)

X. Chen, Z. Zeng, H. Wang, and X. Peng, “Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes,” Nano Res. 8(7), 2251–2260 (2015).
[Crossref]

Nat. Commun. (1)

S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, and M. Leutenegger, “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).
[Crossref] [PubMed]

Nat. Methods (2)

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods 3(10), 793–796 (2006).
[Crossref] [PubMed]

P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. Gustafsson, “Super-resolution video microscopy of live cells by structured illumination,” Nat. Methods 6(5), 339–342 (2009).
[Crossref] [PubMed]

Opt. Express (2)

Opt. Lett. (2)

Opt. Nanoscopy (2)

T. Dertinger, J. Xu, O. F. Naini, R. Vogel, and S. Weiss, “SOFI-based 3D superresolution sectioning with a widefield microscope,” Opt. Nanoscopy 1(1), 2 (2012).
[Crossref] [PubMed]

S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, and T. Lasser, “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI),” Opt. Nanoscopy 1(1), 4 (2012).
[Crossref]

Proc. Natl. Acad. Sci. U.S.A. (1)

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A. 106(52), 22287–22292 (2009).
[Crossref] [PubMed]

Sci. Rep. (1)

Z. Zeng, X. Chen, H. Wang, N. Huang, C. Shan, H. Zhang, J. Teng, and P. Xi, “Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging,” Sci. Rep. 5, 8359 (2015).
[Crossref] [PubMed]

Science (2)

D. Li, L. Shao, B. C. Chen, X. Zhang, M. Zhang, B. Moses, D. E. Milkie, J. R. Beach, J. A. Hammer, M. Pasham, T. Kirchhausen, M. A. Baird, M. W. Davidson, P. Xu, and E. Betzig, “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics,” Science 349(6251), aab3500 (2015).
[Crossref] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science 313(5793), 1642–1645 (2006).
[Crossref] [PubMed]

Other (3)

C. S. Burrus, R. A. Gopinath, and H. Guo, Introduction to Wavelets and Wavelet. Transforms: A Primer (Prentice Hall, 1998)

J. P. Antoine, R. Murenzi, P. Vandergheynst, and S. T. Ali, Two-Dimensional Wavelets and their Relatives (Cambridge U. Press, 2004)

M. Leutenegger, Balanced SOFI toolbox, http://documents.epfl.ch/users/l/le/leuteneg/www/BalancedSOFI/index.html

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Figures (6)

Fig. 1
Fig. 1 Raw time series in four individual pixels from 500 frames of raw images. (a) and (b) are from two adjoining pixels inside an isolated QDs, and (c) and (d) are from two adjoining pixels in the non-blinking background
Fig. 2
Fig. 2 Fluctuating signals extracted from time series presented in Fig. 1 with modified algorithm. Figure 2(a) and (b) are from two adjoining pixels inside an isolated PSF of QDs, and Fig. 2(c) and (d) are from two adjoining pixels in the background.
Fig. 3
Fig. 3 The evolution of the ratio of background to signal (B/S) cross covariance over the number of raw images, (a) Mean subtracting algorithm, (b) Modified algorithm
Fig. 4
Fig. 4 Simulated QD raw images and SOFI images. (a) The centers of the simulated QDs. (b) A typical frame of the simulated raw images. (c) Averaged image of 50 simulated raw images. (d) The 2nd-order SOFI image with 50 raw images using publicly available software [23]. (e) The 2nd-order modified SOFI image without deconvolution or background subtraction. (f) Histogram of FWHM for conventional SOFI and modified SOFI
Fig. 5
Fig. 5 Intensity profiles of line 1 and 2 in Fig. 4(c), (d), and (e) as marked in Fig. 4 (a). (a). Intensity profiles of line 1 in Fig. 4 (c), (d), and (e). (b) Intensity profile of line 2 in Fig. 4 (c), (d), and (e).
Fig. 6
Fig. 6 Comparison between averaged raw image and the modified 4th-order SOFI results of the microtubule network of immunostained Hela cell. (a) Averaged wide-field microscopy image. (b) The modified 4th-order SOFI results with 25 frames of raw images. (c), (d) Magnified views in areas marked in (a) and (b). (e) Profiles of line 1 and 2 marked in (c) and (d). (f) Histogram of FWHMs of PSF in the modified 4th-order SOFI image.

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