Photoacoustic microscopy (PAM) is dominantly sensitive to the endogenous optical absorption compared with the confocal microscopy which images with scattering photons. PAM has similar structure such as optical transportation system, the optical scanning, and light source with the laser scanning confocal microscopy (LSCM). In order to match the PAM with LSCM, a special design microcavity photoacoustic (PA) transducer with high sensitivity is developed to detect the photoacoustic signals induced by modulated continuous wave (CW) laser. By employing a microcavity PA transducer, a PAM can be integrated with LSCM. Thus a simultaneous multimodal imaging can be obtained with the same laser source and optical system. The lateral resolutions of the PAM and the LSCM are both tested to be better than 1.25μm. Then subcellular multimodal imaging can be achieved. Images from the two modes are corresponding with each other but functionally complementary. Combining PAM and LSCM provides more comprehensive information for the cytological test. This technique is demonstrated for imaging red-blood cells and meristematic cells.

©2011 Optical Society of America

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2010 (5)

2009 (1)

2008 (1)

2007 (1)

Blumenkranz, M. S.

Bodapati, S.

Chen, Y.-S.

Chen, Z.

de la Zerda, A.

Dollberg, Y.

Emelianov, S.

Gambhir, S. S.

He, Y.

Homan, K.

Jiao, S.

Khuri-Yakub, B. T.

Kim, S.

Liu, H.

Liu, T.

Mallidi, S.

Maslov, K.

Moshfeghi, D. M.

Paulus, Y. M.

Puliafito, C. A.

Tang, Z.

Teed, R.

Wan, W.

Wang, B.

Wang, J.

Wang, L. V.

Wei, Q.

Wei, Y.

Xie, Z.

Xing, D.

Yang, S.

Yuan, Y.

Zhang, C.

Zhang, H.

Zhang, H. F.

Opt. Express (3)

Opt. Lett. (5)

Other (1)

A. Rosencwaig, Photoacoustics And Photoacoustic Spectroscopy pp:33–34 (1980).

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Figures (6)

Fig. 1
Fig. 1 Schematic of microcavity photoacoustic transducer.
Fig. 2
Fig. 2 Schematic of multimodal cellular imaging system with integrated PAM and LSCM setup.
Fig. 3
Fig. 3 (a) image of continue wave photoacoustic signal in oscillograph. (b) image of noise in oscillograph. (c) photoacoustic signal amplitude with different input power.
Fig. 4
Fig. 4 Images of resolution test target RTA-07 (a) image of optical microscopy. (b) a close-up view of group 25. (c) PA signals at the cross sections highlighted by a-a in (b). (d) PAM image using microcavity PA transducer. (e) Image of LSCM.
Fig. 5
Fig. 5 Images of iron deficiency anemia cells:(a) image from microcavity PA transducer. (d) image from laser scanning confocal microscopy.
Fig. 6
Fig. 6 Images of meristematic cell (a) image of optical microscope .(b) PAM image using microcavity PA transducer. (c) laser scanning confocal microscope image of meristematic cell. CN: cell nucleus.