Abstract

Tomographic phase microscopy (TPM) is an emerging optical microscopic technique for bioimaging. TPM uses digital holographic measurements of complex scattered fields to reconstruct three-dimensional refractive index (RI) maps of cells with diffraction-limited resolution by solving inverse scattering problems. In this paper, we review the developments of TPM from the fundamental physics to its applications in bioimaging. We first provide a comprehensive description of the tomographic reconstruction physical models used in TPM. The RI map reconstruction algorithms and various regularization methods are discussed. Selected TPM applications for cellular imaging, particularly in hematology, are reviewed. Finally, we examine the limitations of current TPM systems, propose future solutions, and envision promising directions in biomedical research.

© 2017 Optical Society of America

Holographic tomography: techniques and biomedical applications [Invited]

Vinoth Balasubramani, Arkadiusz Kuś, Han-Yen Tu, Chau-Jern Cheng, Maria Baczewska, Wojciech Krauze, and Małgorzata Kujawińska
Appl. Opt. 60(10) B65-B80 (2021)

Spatial light interference microscopy: principle and applications to biomedicine

Xi Chen, Mikhail E. Kandel, and Gabriel Popescu
Adv. Opt. Photon. 13(2) 353-425 (2021)

Diffraction phase microscopy: principles and applications in materials and life sciences

Basanta Bhaduri, Chris Edwards, Hoa Pham, Renjie Zhou, Tan H. Nguyen, Lynford L. Goddard, and Gabriel Popescu
Adv. Opt. Photon. 6(1) 57-119 (2014)

Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging

Kyoohyun Kim, Wei Sun Park, Sangchan Na, Sangbum Kim, Taehong Kim, Won Do Heo, and YongKeun Park
Biomed. Opt. Express 8(12) 5688-5697 (2017)

Limited-angle tomographic phase microscopy utilizing confocal scanning fluorescence microscopy

Rongli Guo, Itay Barnea, and Natan T. Shaked
Biomed. Opt. Express 12(4) 1869-1881 (2021)