Abstract

Proper understanding of Raman spectroscopic signals from biological samples requires the quantification of internal signal absorption and its effect on the Raman spectra detected outside the samples under study. In this paper, we describe an efficient Monte Carlo method to simulate Raman scattering in biological tissues and solutions and compare the findings with experimental results obtained in samples with different absorber concentrations and optical properties. As an illustrative example, we focus on solutions of beta-carotene (bCar) in ethanol with different concentrations of absorber (ink) added. We find good agreement between simulation and experiment, thus indicating a way to quantify the influence of internal signal absorption in Raman measurements.

© 2019 Optical Society of America

Simulation of Raman scattering including detector parameters and sampling volume

Ilya Krasnikov, Alexey Seteikin, Ann-Kathrin Kniggendorf, Merve Meinhardt-Wollweber, and Bernhard Roth
J. Opt. Soc. Am. A 34(12) 2138-2144 (2017)

Two efficient approaches for modeling of Raman scattering in homogeneous turbid media

Ilya Krasnikov, Christian Suhr, Alexey Seteikin, Bernhard Roth, and Merve Meinhardt-Wollweber
J. Opt. Soc. Am. A 33(3) 426-433 (2016)

Influence of tissue absorption and scattering on the depth dependent sensitivity of Raman fiber probes investigated by Monte Carlo simulations

Carina Reble, Ingo Gersonde, Chad A. Lieber, and Jürgen Helfmann
Biomed. Opt. Express 2(3) 520-533 (2011)

Efficient Monte Carlo simulation of confocal microscopy in biological tissue

J. M. Schmitt and K. Ben-Letaief
J. Opt. Soc. Am. A 13(5) 952-961 (1996)

Accelerated Monte Carlo models to simulate fluorescence spectra from layered tissues

Johannes Swartling, Antonio Pifferi, Annika M. K. Enejder, and Stefan Andersson-Engels
J. Opt. Soc. Am. A 20(4) 714-727 (2003)