A Collaborative Study of Cary Spectrophotometers*

J. M. Vandenbelt

doi:10.1364/JOSA.44.000641

Journal of the Optical Society of America
Vol. 44,
Issue 8,
pp. 641-643
(1954)
•https://doi.org/10.1364/JOSA.44.000641

A Collaborative Study of Cary Spectrophotometers

J. M. Vandenbelt

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J. M. Vandenbelt, "A Collaborative Study of Cary Spectrophotometers*," J. Opt. Soc. Am. 44, 641-643 (1954)

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Abstract

The absorbance curves of three certified glass filters were determined in the visible range by twelve Cary spectrophotometers used in pharmaceutical laboratories. The means of values obtained for the filters are in good agreement with the certification values. Individual instrumental differences are usually minor.

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Instrument serial No.	Wavelength (mμ)
Instrument serial No.	350	400	450	500	550	600	650	700	750
25 ^a	1.377	1.660	1.412	0.700	0.310	0.159	0.128	0.120	0.125
37 ^a	1.378	1.670	1.415	0.700	0.299	0.154	⋯	⋯	⋯
52 ^a	⋯	1.660	1.431	0.714	0.310	0.157	0.118	0.115	0.128
67 ^c	1.380	1.664	1.400	0.685	0.282	0.150	0.117	0.120	0.128
68 ^b	1.389	1.678	1.401	0.690	0.288	0.146	0.116	0.117	0.127
81 ^a	⋯	1.718	1.434	0.678	0.283	0.146	0.118	0.118	0.130
101 ^c	1.385	1.684	1.400	0.677	0.286	0.151	0.122	0.120	0.131
102 ^a	1.390	1.689	1.415	0.695	0.289	0.151	0.121	0.122	0.139
107 ^b	1.352	1.690	1.375	0.671	0.284	0.150	0.120	0.120	⋯
113 ^c	1.386	1.679	1.389	0.688	0.296	0.150	0.123	0.123	0.137
123 ^a	1.358	1.678	1.390	0.693	0.293	0.148	0.118	0.118	0.133
129 ^b	1.379	1.670	1.397	0.691	0.297	0.150	0.122	0.121	0.127
Mean	1.377	1.678	1.405	0.690	0.293	0.151	0.120	0.119	0.131
Median	1.380	1.678	1.400	0.690	0.291	0.150	0.120	0.120	0.129
A of filter^d	⋯	1.65	1.39	0.690	0.295	0.154	0.123	0.122	0.137
σ_A	0.011	0.015	0.017	0.011	0.009	0.004	0.003	0.002	0.004

Instrument serial No.	Wavelength (mμ)
Instrument serial No.	350	400	450	500	550	600	650	700	750
25 ^a	0.073	0.042	0.106	0.549	1.440	2.126	2.122	0.328	(0.092)
37 ^a	0.078	0.045	0.102	0.554	1.424	2.161	2.130	0.255	0.042
52 ^a	⋯	0.051	0.101	0.532	1.481	2.203	2.172	0.316	0.047
67 ^c	0.075	0.050	0.110	0.553	1.505	2.142	2.163	0.214	0.044
68 ^b	0.074	0.038	0.102	0.569	1.400	2.140	2.092	0.217	0.039
81 ^a	⋯	0.055	0.109	0.568	1.437	2.156	2.115	0.216	0.049
101 ^c	0.075	0.049	0.102	0.570	1.377	2.144	2.081	0.207	0.042
102 ^a	0.070	0.046	0.107	0.577	1.361	2.152	2.088	0.178	0.042
107 ^b	0.072	0.048	0.109	0.603	1.302	2.120	2.062	⋯	⋯
113 ^c	0.079	0.045	0.104	0.568	1.407	2.131	2.102	0.217	0.051
123 ^a	0.071	0.050	0.111	0.580	1.382	2.131	2.090	0.207	0.044
129 ^b	0.074	0.046	0.103	0.565	1.405	2.148	2.114	0.244	0.039
Mean	0.074	0.047	0.106	0.566	1.411	2.145	2.110	0.236	0.044
Median	0.074	0.047	0.105	0.568	1.406	2.143	2.108	0.217	0.044
A of filter^d	⋯	0.053	0.116	0.57	1.42	2.14	2.10	0.235	0.041
σ_A	0.003	0.004	0.003	0.017	0.052	0.021	0.031	0.045	0.015

Instrument serial No.	Wavelength (mμ)
Instrument serial No.	350	400	450	500	550	600	650	700	750
25 ^a	0.158	0.048	0.041	0.056	0.172	0.419	0.719	0.982	1.117
37 ^a	0.146	0.052	0.042	0.057	0.181	0.440	0.772	1.043	1.180
52 ^a	⋯	0.064	0.052	0.062	0.175	0.426	0.751	1.035	1.193
67 ^c	0.154	0.049	0.046	0.058	0.177	0.451	0.768	1.043	1.189
68 ^b	0.145	0.050	0.033	0.056	0.181	0.452	0.781	1.062	1.203
81 ^a	⋯	0.064	0.049	0.063	0.184	0.449	0.780	1.047	1.185
101 ^c	0.157	0.059	0.048	0.061	0.182	0.442	0.779	1.056	1.200
102 ^a	0.153	0.054	0.044	0.060	0.188	0.456	0.795	1.075	1.218
107 ^b	0.143	0.054	0.044	0.065	0.197	0.462	0.790	1.070	⋯
113 ^c	0.156	0.057	0.040	0.060	0.179	0.459	0.784	1.060	1.193
123 ^a	0.144	0.053	0.042	0.062	0.182	0.452	0.788	1.060	1.205
129 ^b	0.158	0.059	0.045	0.064	0.179	0.443	0.773	1.044	1.194
Mean	0.151	0.055	0.044	0.060	0.181	0.446	0.773	1.049	1.189
Median	0.154	0.054	0.044	0.060	0.181	0.450	0.780	1.052	1.193
A of filter^d	⋯	0.060	0.047	0.064	0.183	0.442	0.770	1.053	1.207
σ_A	0.006	0.005	0.004	0.003	0.006	0.012	0.020	0.022	0.024

Instrument serial No.	HT yellow	Cobalt blue
Instrument serial No.	max. 415 mμ	Max. 542 mμ	Min. 563 mμ	Max. 594 mμ	Min. 630 mμ	Max. 643 mμ
25 ^a	1.722	1.495	1.206	2.167	2.001	2.142
37 ^a	1.728	1.513	1.190	2.188	2.013	2.174
52 ^a	1.725	1.523	1.196	2.203	2.025	2.174
67 ^c	1.715	1.506	1.178	2.193	2.009	2.170
68 ^b	1.728	2.515	1.195	2.201	2.016	2.167
81 ^a	1.746	1.559	1.186	2.213	2.024	2.190
101 ^c	1.729	1.515	1.187	2.198	2.014	2.176
102 ^a	1.737	1.526	1.198	2.215	2.028	2.188
107 ^b	1.726	1.519	1.194	2.217	2.028	2.184
113 ^c	1.730	1.515	1.195	2.206	2.019	2.184
123 ^a	1.727	1.518	1.192	2.207	2.019	2.184
129 ^b	1.721	1.512	1.184	2.195	2.012	2.175
Mean	1.728	1.518	1.192	2.200	2.017	2.176
Median	1.728	1.515	1.193	2.202	2.017	2.176
A of filter^d	1.71	1.52	1.195	2.20	2.01	2.16
σ_A	0.008	0.015	0.007	0.013	0.008	0.012

Instrument serial No.	Wavelength (mμ)
Instrument serial No.	350	400	450	500	550	600	650	700	750
25 ^a	1.377	1.660	1.412	0.700	0.310	0.159	0.128	0.120	0.125
37 ^a	1.378	1.670	1.415	0.700	0.299	0.154	⋯	⋯	⋯
52 ^a	⋯	1.660	1.431	0.714	0.310	0.157	0.118	0.115	0.128
67 ^c	1.380	1.664	1.400	0.685	0.282	0.150	0.117	0.120	0.128
68 ^b	1.389	1.678	1.401	0.690	0.288	0.146	0.116	0.117	0.127
81 ^a	⋯	1.718	1.434	0.678	0.283	0.146	0.118	0.118	0.130
101 ^c	1.385	1.684	1.400	0.677	0.286	0.151	0.122	0.120	0.131
102 ^a	1.390	1.689	1.415	0.695	0.289	0.151	0.121	0.122	0.139
107 ^b	1.352	1.690	1.375	0.671	0.284	0.150	0.120	0.120	⋯
113 ^c	1.386	1.679	1.389	0.688	0.296	0.150	0.123	0.123	0.137
123 ^a	1.358	1.678	1.390	0.693	0.293	0.148	0.118	0.118	0.133
129 ^b	1.379	1.670	1.397	0.691	0.297	0.150	0.122	0.121	0.127
Mean	1.377	1.678	1.405	0.690	0.293	0.151	0.120	0.119	0.131
Median	1.380	1.678	1.400	0.690	0.291	0.150	0.120	0.120	0.129
A of filter^d	⋯	1.65	1.39	0.690	0.295	0.154	0.123	0.122	0.137
σ_A	0.011	0.015	0.017	0.011	0.009	0.004	0.003	0.002	0.004

Abstract

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Tables (4)

Journal of the Optical Society of America