Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

The Resolution of Two Particles in a Bright Field by Coated Microscope Objectives

Not Accessible

Your library or personal account may give you access

Abstract

A theory is presented for determining the diffraction image of two, identical, opaque or transmitting, uniform, individually unresolved particles of finite area located in a uniform, transmitting surround. Numerical calculations are included for the classical Airy type objective, for center-blocked Airy type objectives and for simplified Sonine type objectives which have been coated so as to produce diffraction patterns having central disks of reduced diameter. It is shown that marked improvement in the resolution of two particles can be expected by such coated objectives. The resolving power for two particles increases rapidly with the area of the particles and has unexpectedly steep dependence upon the relative amplitude and phase transmission of particle and surround. The theory, subject to experimental verification, forms a basis for explaining why a microscope as used with transmitted light can resolve considerably better than is indicated by the Airy limit.

© 1949 Optical Society of America

Full Article  |  PDF Article
More Like This
The Resolving Power of a Coated Objective

Harold Osterberg and J. Ernest Wilkins
J. Opt. Soc. Am. 39(7) 553-557 (1949)

Effect of Particle Size on the Diffraction Image in Microscopy with Narrow-Coned, Axial Illumination

Harold Osterberg and Gilbert E. Pride
J. Opt. Soc. Am. 40(1) 14-22 (1950)

The Measurement of Unresolved, Single Particles of Uniform Thickness by Means of Variable Phase Microscopy*

Harold Osterberg and Gilbert E. Pride
J. Opt. Soc. Am. 40(2) 64-73 (1950)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (3)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (53)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved