Abstract

Corneal lamellar cutting with a blade or femtosecond laser (FSL) is commonly used during refractive surgery and corneal grafts. Surface roughness of the cutting plane influences postoperative visual acuity but is difficult to assess reliably. For the first time, we compared chromatic confocal microscopy (CCM) with scanning electron microscopy, atomic force microscopy (AFM) and focus-variation microscopy (FVM) to characterize surfaces of variable roughness after FSL cutting. The small area allowed by AFM hinders conclusive roughness analysis, especially with irregular cuts. FVM does not always differentiate between smooth and rough surfaces. Finally, CCM allows analysis of large surfaces and differentiates between surface states.

© 2017 Optical Society of America

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  1. K. Stonecipher, T. S. Ignacio, and M. Stonecipher, “Advances in refractive surgery: microkeratome and femtosecond laser flap creation in relation to safety, efficacy, predictability, and biomechanical stability,” Curr. Opin. Ophthalmol. 17(4), 368–372 (2006).
    [Crossref] [PubMed]
  2. C. A. Swinger and J. I. Barraquer, “Keratophakia and keratomileusis--clinical results,” Ophthalmology 88(8), 709–715 (1981).
    [Crossref] [PubMed]
  3. A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
    [Crossref] [PubMed]
  4. Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
    [Crossref] [PubMed]
  5. P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
    [Crossref] [PubMed]
  6. K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
    [Crossref] [PubMed]
  7. O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).
  8. M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
    [Crossref] [PubMed]
  9. S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
    [Crossref] [PubMed]
  10. V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
    [Crossref] [PubMed]
  11. M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
    [Crossref] [PubMed]
  12. M. A. Sarayba, T. S. Ignacio, P. S. Binder, and D. B. Tran, “Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes,” Cornea 26(4), 446–451 (2007).
    [Crossref] [PubMed]
  13. Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
    [Crossref] [PubMed]
  14. Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
    [Crossref] [PubMed]
  15. A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
    [Crossref] [PubMed]
  16. M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
    [PubMed]
  17. M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
    [Crossref] [PubMed]
  18. N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
    [Crossref] [PubMed]
  19. W. Kapłonek, K. Nadolny, and G. M. Królczyk, “The Use of Focus-Variation Microscopy for the Assessment of Active Surfaces of a New Generation of Coated Abrasive Tools,” Meas. Sci. Rev. 16(2), 42–53 (2016).
    [Crossref]
  20. D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
    [Crossref] [PubMed]

2016 (6)

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

W. Kapłonek, K. Nadolny, and G. M. Królczyk, “The Use of Focus-Variation Microscopy for the Assessment of Active Surfaces of a New Generation of Coated Abrasive Tools,” Meas. Sci. Rev. 16(2), 42–53 (2016).
[Crossref]

2015 (3)

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
[Crossref] [PubMed]

2014 (2)

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

2013 (1)

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

2012 (2)

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

2011 (1)

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

2010 (1)

O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).

2008 (1)

Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
[Crossref] [PubMed]

2007 (1)

M. A. Sarayba, T. S. Ignacio, P. S. Binder, and D. B. Tran, “Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes,” Cornea 26(4), 446–451 (2007).
[Crossref] [PubMed]

2006 (1)

K. Stonecipher, T. S. Ignacio, and M. Stonecipher, “Advances in refractive surgery: microkeratome and femtosecond laser flap creation in relation to safety, efficacy, predictability, and biomechanical stability,” Curr. Opin. Ophthalmol. 17(4), 368–372 (2006).
[Crossref] [PubMed]

1981 (1)

C. A. Swinger and J. I. Barraquer, “Keratophakia and keratomileusis--clinical results,” Ophthalmology 88(8), 709–715 (1981).
[Crossref] [PubMed]

Acquart, S.

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Aldossary, M.

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

Ang, H. P.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Asato, H.

K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
[Crossref] [PubMed]

Barberi, R.

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

Barraquer, J. I.

C. A. Swinger and J. I. Barraquer, “Keratophakia and keratomileusis--clinical results,” Ophthalmology 88(8), 709–715 (1981).
[Crossref] [PubMed]

Berendschot, T. T.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

Bergin, C.

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

Bernard, A.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Binder, P. S.

M. A. Sarayba, T. S. Ignacio, P. S. Binder, and D. B. Tran, “Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes,” Cornea 26(4), 446–451 (2007).
[Crossref] [PubMed]

Bowman, R. W.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).

Buratto, L.

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

Cavanagh, H. D.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

Cognasse, F.

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

Courrier, E.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

Culbertson, W. W.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

De Santo, M. P.

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

Delbosc, B.

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Desiderio, G.

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

Dias, J.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

Dickman, M. M.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

Ducoli, P.

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

Dumollard, J. M.

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Flammersfeld, A.

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

Forest, F.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Fuest, M.

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

Gain, P.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Gauthier, A. S.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Goins, K. M.

Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
[Crossref] [PubMed]

He, Z.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Heck, E.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

Hermel, M.

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

Hirayama, Y.

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Hürmeriç, V.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

Igarashi, A.

K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
[Crossref] [PubMed]

Ignacio, T. S.

M. A. Sarayba, T. S. Ignacio, P. S. Binder, and D. B. Tran, “Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes,” Cornea 26(4), 446–451 (2007).
[Crossref] [PubMed]

K. Stonecipher, T. S. Ignacio, and M. Stonecipher, “Advances in refractive surgery: microkeratome and femtosecond laser flap creation in relation to safety, efficacy, predictability, and biomechanical stability,” Curr. Opin. Ophthalmol. 17(4), 368–372 (2006).
[Crossref] [PubMed]

Jones, Y. J.

Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
[Crossref] [PubMed]

Jullienne, R.

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

Jumelle, C.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

Kamiya, K.

K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
[Crossref] [PubMed]

Kaplonek, W.

W. Kapłonek, K. Nadolny, and G. M. Królczyk, “The Use of Focus-Variation Microscopy for the Assessment of Active Surfaces of a New Generation of Coated Abrasive Tools,” Meas. Sci. Rev. 16(2), 42–53 (2016).
[Crossref]

Kobashi, H.

K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
[Crossref] [PubMed]

Królczyk, G. M.

W. Kapłonek, K. Nadolny, and G. M. Królczyk, “The Use of Focus-Variation Microscopy for the Assessment of Active Surfaces of a New Generation of Coated Abrasive Tools,” Meas. Sci. Rev. 16(2), 42–53 (2016).
[Crossref]

Kuerten, D.

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

Lakshman, N.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

Liu, Y. C.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Lombardo, G.

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

Lombardo, M.

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

Lwin, N. C.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Majo, F.

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

McCulley, J. P.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).

Mehta, J. S.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Montard, R.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Mootha, V. V.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).

Moy, V. T.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

Muftuoglu, O.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).

Mullins, R.

Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
[Crossref] [PubMed]

Nadolny, K.

W. Kapłonek, K. Nadolny, and G. M. Królczyk, “The Use of Focus-Variation Microscopy for the Assessment of Active Surfaces of a New Generation of Coated Abrasive Tools,” Meas. Sci. Rev. 16(2), 42–53 (2016).
[Crossref]

Nicolas, M.

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

Nuijts, R. M.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

Ogawa, A.

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Peoc’h, M.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

Peoch, M.

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Perrache, C.

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

Petroll, W. M.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

Plange, N.

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

Plum, W. J.

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

Prasher, P.

O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).

Riau, A. K.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Roy, S.

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

Rütten, S.

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

Salla, S.

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

Sarayba, M. A.

M. A. Sarayba, T. S. Ignacio, P. S. Binder, and D. B. Tran, “Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes,” Cornea 26(4), 446–451 (2007).
[Crossref] [PubMed]

Satake, Y.

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Schiano Lomoriello, D.

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

Schnitzler, A. C.

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

Schuchard, Y.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

Serrao, S.

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

Shimazaki, J.

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Shimazaki-Den, S.

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Shimizu, K.

K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
[Crossref] [PubMed]

Shousha, M. A.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

Skeie, J. M.

Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
[Crossref] [PubMed]

Steijger-Vermaat, P.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

Stonecipher, K.

K. Stonecipher, T. S. Ignacio, and M. Stonecipher, “Advances in refractive surgery: microkeratome and femtosecond laser flap creation in relation to safety, efficacy, predictability, and biomechanical stability,” Curr. Opin. Ophthalmol. 17(4), 368–372 (2006).
[Crossref] [PubMed]

Stonecipher, M.

K. Stonecipher, T. S. Ignacio, and M. Stonecipher, “Advances in refractive surgery: microkeratome and femtosecond laser flap creation in relation to safety, efficacy, predictability, and biomechanical stability,” Curr. Opin. Ophthalmol. 17(4), 368–372 (2006).
[Crossref] [PubMed]

Sutphin, J. E.

Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
[Crossref] [PubMed]

Swinger, C. A.

C. A. Swinger and J. I. Barraquer, “Keratophakia and keratomileusis--clinical results,” Ophthalmology 88(8), 709–715 (1981).
[Crossref] [PubMed]

Tan, D. T.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Tan, N. Y.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Thuret, G.

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

Tomida, D.

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Tran, D. B.

M. A. Sarayba, T. S. Ignacio, P. S. Binder, and D. B. Tran, “Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes,” Cornea 26(4), 446–451 (2007).
[Crossref] [PubMed]

Tschuor, P.

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

van den Biggelaar, F. J.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

van Marion, F. W.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

van Maris, M. P.

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

Varga, Z.

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

Verity, S. M.

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

Walter, P.

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

Yam, G. H.

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

Yamaguchi, T.

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Yau, C. B.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

Yoo, S. H.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

Ziebarth, N. M.

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

Acta Ophthalmol. (1)

M. Fuest, S. Salla, M. Hermel, W. J. Plum, S. Rütten, N. Plange, D. Kuerten, A. C. Schnitzler, and P. Walter, “Gebauer SLc Original and Moria One-Use Plus automated microkeratomes for ultrathin Descemet’s stripping automated endothelial keratoplasty preparation,” Acta Ophthalmol. 94(8), e731–e737 (2016).
[Crossref] [PubMed]

Cornea (5)

A. Bernard, Z. He, F. Forest, A. S. Gauthier, M. Peoch, J. M. Dumollard, S. Acquart, R. Montard, B. Delbosc, P. Gain, and G. Thuret, “Femtosecond laser cutting of multiple thin corneal stromal lamellae for endothelial bioengineering,” Cornea 34(2), 218–224 (2015).
[Crossref] [PubMed]

M. Lombardo, M. P. De Santo, G. Lombardo, D. Schiano Lomoriello, G. Desiderio, P. Ducoli, R. Barberi, and S. Serrao, “Surface quality of femtosecond dissected posterior human corneal stroma investigated with atomic force microscopy,” Cornea 31(12), 1369–1375 (2012).
[Crossref] [PubMed]

V. V. Mootha, E. Heck, S. M. Verity, W. M. Petroll, N. Lakshman, O. Muftuoglu, R. W. Bowman, J. P. McCulley, and H. D. Cavanagh, “Comparative study of descemet stripping automated endothelial keratoplasty donor preparation by Moria CBm microkeratome, horizon microkeratome, and Intralase FS60,” Cornea 30(3), 320–324 (2011).
[Crossref] [PubMed]

M. A. Sarayba, T. S. Ignacio, P. S. Binder, and D. B. Tran, “Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes,” Cornea 26(4), 446–451 (2007).
[Crossref] [PubMed]

Y. J. Jones, K. M. Goins, J. E. Sutphin, R. Mullins, and J. M. Skeie, “Comparison of the femtosecond laser (IntraLase) versus manual microkeratome (Moria ALTK) in dissection of the donor in endothelial keratoplasty: initial study in eye bank eyes,” Cornea 27(1), 88–93 (2008).
[Crossref] [PubMed]

Curr. Eye Res. (2)

M. Fuest, S. Salla, P. Walter, N. Plange, D. Kuerten, A. Flammersfeld, and M. Hermel, “Comparison of Gebauer SLc and Moria CBm Carriazo-Barraquer ALK Microkeratomes for Descemet’s Stripping Automated Endothelial Keratoplasty Preparation,” Curr. Eye Res. 41(3), 343–349 (2016).
[PubMed]

Z. Varga, C. Bergin, S. Roy, M. Nicolas, P. Tschuor, and F. Majo, “Scanning Electronic Microscopy Evaluation of the Roughness of the Stromal Bed After Deep Corneal Cut with the LDV Femtosecond Laser (Z6) (Ziemer) and the ONE Microkeratome (Moria),” Curr. Eye Res. 41(10), 1302–1309 (2016).
[Crossref] [PubMed]

Curr. Opin. Ophthalmol. (1)

K. Stonecipher, T. S. Ignacio, and M. Stonecipher, “Advances in refractive surgery: microkeratome and femtosecond laser flap creation in relation to safety, efficacy, predictability, and biomechanical stability,” Curr. Opin. Ophthalmol. 17(4), 368–372 (2006).
[Crossref] [PubMed]

Invest. Ophthalmol. Vis. Sci. (3)

Z. He, F. Forest, A. Bernard, A. S. Gauthier, R. Montard, M. Peoc’h, C. Jumelle, E. Courrier, C. Perrache, P. Gain, and G. Thuret, “Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts,” Invest. Ophthalmol. Vis. Sci. 57(15), 6639–6651 (2016).
[Crossref] [PubMed]

M. M. Dickman, M. P. van Maris, F. W. van Marion, Y. Schuchard, P. Steijger-Vermaat, F. J. van den Biggelaar, T. T. Berendschot, and R. M. Nuijts, “Surface metrology and 3-dimensional confocal profiling of femtosecond laser and mechanically dissected ultrathin endothelial lamellae,” Invest. Ophthalmol. Vis. Sci. 55(8), 5183–5190 (2014).
[Crossref] [PubMed]

A. K. Riau, Y. C. Liu, N. C. Lwin, H. P. Ang, N. Y. Tan, G. H. Yam, D. T. Tan, and J. S. Mehta, “Comparative study of nJ- and μJ-energy level femtosecond lasers: evaluation of flap adhesion strength, stromal bed quality, and tissue responses,” Invest. Ophthalmol. Vis. Sci. 55(5), 3186–3194 (2014).
[Crossref] [PubMed]

J. Cataract Refract. Surg. (2)

S. Serrao, L. Buratto, G. Lombardo, M. P. De Santo, P. Ducoli, and M. Lombardo, “Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted laser in situ keratomileusis,” J. Cataract Refract. Surg. 38(8), 1453–1459 (2012).
[Crossref] [PubMed]

N. M. Ziebarth, J. Dias, V. Hürmeriç, M. A. Shousha, C. B. Yau, V. T. Moy, W. W. Culbertson, and S. H. Yoo, “Quality of corneal lamellar cuts quantified using atomic force microscopy,” J. Cataract Refract. Surg. 39(1), 110–117 (2013).
[Crossref] [PubMed]

JAMA Ophthalmol. (1)

P. Gain, R. Jullienne, Z. He, M. Aldossary, S. Acquart, F. Cognasse, and G. Thuret, “Global Survey of Corneal Transplantation and Eye Banking,” JAMA Ophthalmol. 134(2), 167–173 (2016).
[Crossref] [PubMed]

Jpn. J. Ophthalmol. (1)

D. Tomida, T. Yamaguchi, A. Ogawa, Y. Hirayama, S. Shimazaki-Den, Y. Satake, and J. Shimazaki, “Effects of corneal irregular astigmatism on visual acuity after conventional and femtosecond laser-assisted Descemet’s stripping automated endothelial keratoplasty,” Jpn. J. Ophthalmol. 59(4), 216–222 (2015).
[Crossref] [PubMed]

Meas. Sci. Rev. (1)

W. Kapłonek, K. Nadolny, and G. M. Królczyk, “The Use of Focus-Variation Microscopy for the Assessment of Active Surfaces of a New Generation of Coated Abrasive Tools,” Meas. Sci. Rev. 16(2), 42–53 (2016).
[Crossref]

Ophthalmology (2)

C. A. Swinger and J. I. Barraquer, “Keratophakia and keratomileusis--clinical results,” Ophthalmology 88(8), 709–715 (1981).
[Crossref] [PubMed]

O. Muftuoglu, P. Prasher, R. W. Bowman, J. P. McCulley, and V. V. Mootha, “Corneal higher-order aberrations after Descemet's stripping automated endothelial keratoplasty,” Ophthalmology 117(5), 878–884 (2010).

PLoS One (1)

K. Kamiya, H. Asato, K. Shimizu, H. Kobashi, and A. Igarashi, “Effect of Intraocular Forward Scattering and Corneal Higher-Order Aberrations on Visual Acuity after Descemet’s Stripping Automated Endothelial Keratoplasty,” PLoS One 10(6), e0131110 (2015).
[Crossref] [PubMed]

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Figures (6)

Fig. 1
Fig. 1 Flow chart of experiments. Steps 1 and 2 were designed to study the influence on roughness measurement of stabilizing the samples by dehydration and metallization. In step 3, the three quantitative microscopy methods were compared.
Fig. 2
Fig. 2 Method of roughness calculation. Example of an X profile and a Y profile from a height map (black line). 1/ Mean straight lines (white lines) were generated for each X and Y profile. 2/ Each profile was separated into five sampling lengths. 3/ RRMS was calculated for each segment, with the n being the number of Z-heights measured between the X (or Y) profile and its associated mean straight line. 4/ For each X or Y profile, the profile-RRMS was calculated as the mean RRMS of its five sampling lengths. 5/ X-RRMS and Y-RRMS were calculated as the mean of all profiles along the X or Y axis. 6/ Lastly, total-RRMS was calculated as the mean of X-mean RRMS and Y-mean RRMS.
Fig. 3
Fig. 3 Comparison of environmental and standard scanning electron microscopy using a surface state obtained after corneal cutting with a femtosecond laser. Both images are displayed with the same magnification. Scale bar = 100 µm.
Fig. 4
Fig. 4 Direct comparison of scanning electron microscopy and the three quantitative methods (atomic force microscopy, focus-variation microscopy, chromatic confocal microscopy). Lamellae with low and high roughness were compared with the naturally smooth surface of the Bowman membrane (BM, control). 10x10 um2 areas selected at random were compared. The surface of the same lamellae appeared different on the height-maps provided by the three microscopes, especially in the high-roughness group. Each height-map was presented with its roughness value expressed in nm. For the same sample, roughness values varied from 1 to 4 according to the measurement methods. Statistics: Kruskal-Wallis tests. P values were indicated for each row and each column. Scale bar = 5 µm.
Fig. 5
Fig. 5 Influence of area observed by focus-variation (FVM) and chromatic confocal microscopy (CCM) on roughness measurement. 3D maps of lamellae with smooth, low-roughness and high-roughness surfaces. For each lamella, areas of 100x100 µm2, 300x300µm2 and 1000x1000 µm2 were characterized by FVM and CCM. Each height-map is associated with its roughness value in nm.
Fig. 6
Fig. 6 Surface roughness mean and error of three corneal lamellar surfaces (smooth, low/high roughness) per surface area (100x100 µm2, 300x300 µm2, 1000x1000 µm2) and characterization technique (focus-variation microscopy (FVM) or chromatic confocal microscopy (CCM)).

Tables (1)

Tables Icon

Table 1 Main characteristics of the four microscopy methods used to measure surface roughness.

Equations (1)

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RMS= 1 n il a z i 2

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