Abstract

We present for the first time one-to-one correspondence between standard hematoxylin/eosin (H&E) stained tissue sections and stimulated Raman histology (SRH) - a label-free technique in which stimulated Raman scattering (SRS) and second harmonic generation (SHG) are combined to generate virtual H&E images. Experiments were performed on both human thin cryogenic slides from the gastrointestinal tract (GI) and thick freshly excised biopsies from endoscopic surgery. Results on cryogenic slides evidenced an excellent agreement between SRH and H&E images while the ones on biopsies established the relevance of SRH for rapid intraoperative histology to assist in surgical decision making.

© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Full Article  |  PDF Article
OSA Recommended Articles
Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins

Michael G. Giacomelli, Beverly E. Faulkner-Jones, Lucas C. Cahill, Tadayuki Yoshitake, Daihung Do, and James G. Fujimoto
Biomed. Opt. Express 10(8) 4249-4260 (2019)

Rapid, large-scale stimulated Raman histology with strip mosaicing and dual-phase detection

Bohan Zhang, Mengxiong Sun, Yifan Yang, Lingchao Chen, Xiang Zou, Tian Yang, Yingqi Hua, and Minbiao Ji
Biomed. Opt. Express 9(6) 2604-2613 (2018)

Slide-free virtual histochemistry (Part II): detection of field cancerization

Sixian You, Yi Sun, Eric J. Chaney, Youbo Zhao, Jianxin Chen, Stephen A. Boppart, and Haohua Tu
Biomed. Opt. Express 9(11) 5253-5268 (2018)

References

  • View by:
  • |
  • |
  • |

  1. M. T. Cicerone and C. H. Camp, “Histological coherent Raman imaging: a prognostic review,” Analyst 143(1), 33–59 (2018).
    [Crossref]
  2. H. Rigneault and P. Berto, “Tutorial: Coherent Raman light matter interaction processes,” APL Photonics 3(9), 091101 (2018).
    [Crossref]
  3. D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
    [Crossref]
  4. B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
    [Crossref]
  5. H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
    [Crossref]
  6. Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
    [Crossref]
  7. A. I. Baba and C. Câtoi, Comparative Oncology (The Publishing House of the Romanian Academy, 2007).
  8. C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
    [Crossref]
  9. M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
    [Crossref]
  10. R. He, Y. Xu, L. Zhang, S. Ma, X. Wang, D. Ye, and M. Ji, “Dual-phase stimulated Raman scattering microscopy for real-time two-color imaging,” Optica 4(1), 44–47 (2017).
    [Crossref]
  11. S. Heuke, B. Sarri, X. Audier, and H. Rigneault, “Simultaneous dual-channel stimulated Raman scattering microscopy demultiplexed at distinct modulation frequencies,” Opt. Lett. 43(15), 3582–3585 (2018).
    [Crossref]
  12. S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
    [Crossref]
  13. P. Ferrand, “GPScan.VI: A general-purpose LabVIEW program for scanning imaging or any application requiring synchronous analog voltage generation and data acquisition,” Comput. Phys. Commun. 192, 342–347 (2015).
    [Crossref]
  14. S. Heuke, B. Sarri, A. Lombardini, X. Audier, and H. Rigneault, “Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling,” Opt. Lett. 43(19), 4763–4766 (2018).
    [Crossref]
  15. B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
    [Crossref]
  16. A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
    [Crossref]

2019 (1)

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

2018 (5)

M. T. Cicerone and C. H. Camp, “Histological coherent Raman imaging: a prognostic review,” Analyst 143(1), 33–59 (2018).
[Crossref]

H. Rigneault and P. Berto, “Tutorial: Coherent Raman light matter interaction processes,” APL Photonics 3(9), 091101 (2018).
[Crossref]

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

S. Heuke, B. Sarri, X. Audier, and H. Rigneault, “Simultaneous dual-channel stimulated Raman scattering microscopy demultiplexed at distinct modulation frequencies,” Opt. Lett. 43(15), 3582–3585 (2018).
[Crossref]

S. Heuke, B. Sarri, A. Lombardini, X. Audier, and H. Rigneault, “Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling,” Opt. Lett. 43(19), 4763–4766 (2018).
[Crossref]

2017 (3)

R. He, Y. Xu, L. Zhang, S. Ma, X. Wang, D. Ye, and M. Ji, “Dual-phase stimulated Raman scattering microscopy for real-time two-color imaging,” Optica 4(1), 44–47 (2017).
[Crossref]

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

2015 (1)

P. Ferrand, “GPScan.VI: A general-purpose LabVIEW program for scanning imaging or any application requiring synchronous analog voltage generation and data acquisition,” Comput. Phys. Commun. 192, 342–347 (2015).
[Crossref]

2013 (1)

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

2012 (1)

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

2011 (1)

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

2009 (1)

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

2004 (1)

H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
[Crossref]

Agar, N. Y.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Andresen, E. R.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Audier, X.

Baba, A. I.

A. I. Baba and C. Câtoi, Comparative Oncology (The Publishing House of the Romanian Academy, 2007).

Berto, P.

H. Rigneault and P. Berto, “Tutorial: Coherent Raman light matter interaction processes,” APL Photonics 3(9), 091101 (2018).
[Crossref]

Bian, X. W.

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

Billaudeau, C.

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

Boyle, J.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Brasselet, S.

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

Brustlein, S.

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

Cador, C.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Caillol, F.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Camelo-Piragua, S.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Camp, C. H.

M. T. Cicerone and C. H. Camp, “Histological coherent Raman imaging: a prognostic review,” Analyst 143(1), 33–59 (2018).
[Crossref]

Canonge, R.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Câtoi, C.

A. I. Baba and C. Câtoi, Comparative Oncology (The Publishing House of the Romanian Academy, 2007).

Chen, X.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Cicerone, M. T.

M. T. Cicerone and C. H. Camp, “Histological coherent Raman imaging: a prognostic review,” Analyst 143(1), 33–59 (2018).
[Crossref]

Cui, Y. H.

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

De Jager, P. L.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Fabert, M.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Ferrand, P.

P. Ferrand, “GPScan.VI: A general-purpose LabVIEW program for scanning imaging or any application requiring synchronous analog voltage generation and data acquisition,” Comput. Phys. Commun. 192, 342–347 (2015).
[Crossref]

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

Fisher-Hubbard, A.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Freudiger, C. W.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Garrard, M.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Garton, H. J. L.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Giovannini, M.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Golby, A. J.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Gonzales, L. F.

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

Hayashi, M.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

He, R.

Hervey-Jumper, S. L.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Heth, J. A.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Heuke, S.

Hollon, T. C.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Jass, J. R.

H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
[Crossref]

Ji, C. D.

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

Ji, M.

R. He, Y. Xu, L. Zhang, S. Ma, X. Wang, D. Ye, and M. Ji, “Dual-phase stimulated Raman scattering microscopy for real-time two-color imaging,” Optica 4(1), 44–47 (2017).
[Crossref]

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Ji, M. B.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Johnson, T. D.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Joly, N.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Jones, A. M.

H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
[Crossref]

Kesari, S.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Killory, B. D.

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

Kudlinski, A.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Lau, D.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Lewis, S.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Lieberman, A. P.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Ligon, K. L.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Liu, X.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Lombardini, A.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

S. Heuke, B. Sarri, A. Lombardini, X. Audier, and H. Rigneault, “Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling,” Opt. Lett. 43(19), 4763–4766 (2018).
[Crossref]

Louradour, F.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Ma, S.

Maher, C. O.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Marguet, D.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

McFadden, K. A.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Mytskaniuk, V.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Nakaji, P.

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

Niknafs, Y. S.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Norton, I.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Orringer, D. A.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Ottoboni, L.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Pandian, B.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Pfannl, R.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Philbert, M. A.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Poizat, F.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Ponce, F. A.

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

Ramkissoon, S.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Ramkissoon, S. H.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Rigneault, H.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

H. Rigneault and P. Berto, “Tutorial: Coherent Raman light matter interaction processes,” APL Photonics 3(9), 091101 (2018).
[Crossref]

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

S. Heuke, B. Sarri, A. Lombardini, X. Audier, and H. Rigneault, “Dual-focus stimulated Raman scattering microscopy: a concept for multi-focus scaling,” Opt. Lett. 43(19), 4763–4766 (2018).
[Crossref]

S. Heuke, B. Sarri, X. Audier, and H. Rigneault, “Simultaneous dual-channel stimulated Raman scattering microscopy demultiplexed at distinct modulation frequencies,” Opt. Lett. 43(15), 3582–3585 (2018).
[Crossref]

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

Saar, B. G.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Sagher, O.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Santagata, S.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Sarri, B.

Simon, E.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Sims, J. R.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Sivankutty, S.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Snuderl, M.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Spetzler, R. F.

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

Spino, C.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Talbot, I. C.

H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
[Crossref]

Trautman, J. K.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Ueno, H.

H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
[Crossref]

Venneti, S.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Waeber, C.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Wait, S. D.

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

Walther, N.

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

Wang, X.

Wenger, J.

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Wilkinson, D. A.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Wilkinson, K. H.

H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
[Crossref]

Wojak, J.

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Xiao, H. L.

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

Xie, X. S.

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Xu, X. Y.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Xu, Y.

Ye, D.

Ying, W.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Young, G. S.

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Zeng, Q.

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Zhang, L.

Zhao, H. B.

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

Zhou, Z. H.

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

Analyst (1)

M. T. Cicerone and C. H. Camp, “Histological coherent Raman imaging: a prognostic review,” Analyst 143(1), 33–59 (2018).
[Crossref]

APL Photonics (1)

H. Rigneault and P. Berto, “Tutorial: Coherent Raman light matter interaction processes,” APL Photonics 3(9), 091101 (2018).
[Crossref]

Comput. Phys. Commun. (1)

P. Ferrand, “GPScan.VI: A general-purpose LabVIEW program for scanning imaging or any application requiring synchronous analog voltage generation and data acquisition,” Comput. Phys. Commun. 192, 342–347 (2015).
[Crossref]

Gut (1)

H. Ueno, A. M. Jones, K. H. Wilkinson, J. R. Jass, and I. C. Talbot, “Histological categorisation of fibrotic cancer stroma in advanced rectal cancer,” Gut 53(4), 581–586 (2004).
[Crossref]

J. Biomed. Opt. (1)

S. Brustlein, P. Ferrand, N. Walther, S. Brasselet, C. Billaudeau, D. Marguet, and H. Rigneault, “Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview,” J. Biomed. Opt. 16(2), 021106 (2011).
[Crossref]

J. Cancer (Wyoming, Aust.) (1)

Z. H. Zhou, C. D. Ji, H. L. Xiao, H. B. Zhao, Y. H. Cui, and X. W. Bian, “Reorganized Collagen in the Tumor Microenvironment of Gastric Cancer and Its Association with Prognosis,” J. Cancer (Wyoming, Aust.) 8(8), 1466–1476 (2017).
[Crossref]

Lab. Invest. (1)

C. W. Freudiger, R. Pfannl, D. A. Orringer, B. G. Saar, M. B. Ji, Q. Zeng, L. Ottoboni, W. Ying, C. Waeber, J. R. Sims, P. L. De Jager, O. Sagher, M. A. Philbert, X. Y. Xu, S. Kesari, X. S. Xie, and G. S. Young, “Multicolored stain-free histopathology with coherent Raman imaging,” Lab. Invest. 92(10), 1492–1502 (2012).
[Crossref]

Light: Sci. Appl. (1)

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Sci. Appl. 7(1), 10 (2018).
[Crossref]

Nat. Biomed. Eng. (1)

D. A. Orringer, B. Pandian, Y. S. Niknafs, T. C. Hollon, J. Boyle, S. Lewis, M. Garrard, S. L. Hervey-Jumper, H. J. L. Garton, C. O. Maher, J. A. Heth, O. Sagher, D. A. Wilkinson, M. Snuderl, S. Venneti, S. H. Ramkissoon, K. A. McFadden, A. Fisher-Hubbard, A. P. Lieberman, T. D. Johnson, X. S. Xie, J. K. Trautman, C. W. Freudiger, and S. Camelo-Piragua, “Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy,” Nat. Biomed. Eng. 1(2), 0027 (2017).
[Crossref]

Neurosurgery (1)

B. D. Killory, P. Nakaji, L. F. Gonzales, F. A. Ponce, S. D. Wait, and R. F. Spetzler, “Prospective evaluation of surgical microscope-integrated intraoperative near-infrared indocyanine green angiography during cerebral arteriovenous malformation surgery,” Neurosurgery 65(3), 456–462 (2009).
[Crossref]

Opt. Lett. (2)

Optica (1)

Sci. Rep. (1)

B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, “Fast stimulated Raman and second Harmonic generation imaging for intraoperative gastro-intestinal cancer detection,” Sci. Rep. 9(1), 10052 (2019).
[Crossref]

Sci. Transl. Med. (1)

M. Ji, D. A. Orringer, C. W. Freudiger, S. Ramkissoon, X. Liu, D. Lau, A. J. Golby, I. Norton, M. Hayashi, N. Y. Agar, G. S. Young, C. Spino, S. Santagata, S. Camelo-Piragua, K. L. Ligon, O. Sagher, and X. S. Xie, “Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy,” Sci. Transl. Med. 5(201), 201ra119 (2013).
[Crossref]

Other (1)

A. I. Baba and C. Câtoi, Comparative Oncology (The Publishing House of the Romanian Academy, 2007).

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)

Fig. 1.
Fig. 1. One to one SRH/H&E comparison on a 8 µm thick cryogenic slide of muscosa from human stomach. (a) Built image of SRS2930cm-1 – SRS2845cm-1 displaying the nuclei distribution of the region highlighted with the black squares in the H&E (dashed square) and SRH (solid square) images; (b) SRS image at 2845cm−1 highlighting the cell body distribution of the same region; (c) SHG image providing the collagen distribution of the same region. (d) H&E staining of a larger region (0.6mmx0.6mm) of the same cryogenic slide. (e) SRH image built using (a, b and c) displaying the same extended region as in (d) and demonstrated similar features. Scale bar is 100 µm.
Fig. 2.
Fig. 2. One to one SRH/H&E comparison on a 8µm thick cryogenic slide for normal human colon. (a) SRH image and (b) the H&E corresponding image of the same cryogenic region. (c) SRH image of a wider (1 mm x1 mm) area encapsulated into a larger region of the identical slide stained with H&E. The red square corresponds to (a). Scale bar is 100 µm
Fig. 3.
Fig. 3. One to one SRH/H&E comparison on a 10 µm thick cryogenic slide for cancerous (adenocarcinoma) human colon. (a) SRH image and (b) the H&E corresponding image of the same cryogenic region. (c) SRH image of a wider area (1.2 mm x 0.8 mm) encapsulated into a larger region of the identical slide stained with H&E. The red rectangle corresponds to (a). Scale bar is 100µm.
Fig. 4.
Fig. 4. SRH/HES comparison for critical pathological situations. (a) SRH image of a cancerous omemtum, (b) standard HES of cancerous omemtum from the same patient, (c) SRH image from a thick biopsy sample, imaged directly after its gastric endoscopic excision, of a poorly differentiated adenoma case, (d) standard HES from the same patient as in (c). Scale bar is 100 µm.

Metrics