Abstract

Fiber imaging bundles are widely used as thin, passive image conduits for miniaturized and endoscopic microscopy, particularly for confocal fluorescence imaging. Holographic microscopy through fiber bundles is more challenging; phase conjugation approaches are complex and require extensive calibration. This paper describes how simple inline holographic microscopy can be performed through an imaging bundle using a partially coherent illumination source from a multimode fiber. The sample is imaged in transmission, with the intensity hologram sampled by the bundle and transmitted to a remote camera. The hologram can then be numerically refocused for volumetric imaging, achieving a resolution of approximately 6 µm over a depth range of 1 mm. The scheme does not require any complex prior calibration and hence is insensitive to bending.

© 2020 Optical Society of America

Improved resolution in fiber bundle inline holographic microscopy using multiple illumination sources

Michael R. Hughes and Callum McCall
Biomed. Opt. Express 15(3) 1500-1514 (2024)

Real-timing processing of fiber bundle endomicroscopy images in Python using PyFibreBundle

Michael R. Hughes
Appl. Opt. 62(34) 9041-9050 (2023)

Widefield lensless imaging through a fiber bundle via speckle correlations

Amir Porat, Esben Ravn Andresen, Hervé Rigneault, Dan Oron, Sylvain Gigan, and Ori Katz
Opt. Express 24(15) 16835-16855 (2016)

Inline holographic coherent anti-Stokes Raman microscopy

Qian Xu, Kebin Shi, Haifeng Li, Kerkil Choi, Ryoichi Horisaki, David Brady, Demetri Psaltis, and Zhiwen Liu
Opt. Express 18(8) 8213-8219 (2010)

Achieving stability in remote holography using flexible multimode image bundles

T. D. Dudderar, J. A. Gilbert, and A. J. Boehnfein
Appl. Opt. 22(7) 1000-1005 (1983)