Abstract

Transmittance and fluorescence optical projection tomography can offer high-resolution and high-contrast visualization of whole biological specimens; however, applications are limited to samples exhibiting minimal light scattering. Our previous work demonstrated that angular-domain techniques permitted imaging of ${\sim}{1}\;{\rm cm}$ diameter noncleared lymph nodes because of their low scattering nature. Here, an angle-restricted transmittance/fluorescence system is presented and characterized in terms of geometric and fluorescence concentration reconstruction accuracy as well as spatial resolution, depth of focus, and fluorescence limits of detection. Using lymph node mimicking phantoms, results demonstrated promising detection and localization capabilities relevant for clinical lymph node applications.

© 2020 Optical Society of America

Model-based system matrix for iterative reconstruction in sub-diffuse angular-domain fluorescence optical projection tomography

Veronica C. Torres, Chengyue Li, Jovan G. Brankov, and Kenneth M. Tichauer
Biomed. Opt. Express 12(3) 1248-1262 (2021)

Comparison of time- and angular-domain scatter rejection in mesoscopic optical projection tomography: a simulation study

L. Sinha, F. Massanes, V. C. Torres, C. Li, K. M. Tichauer, and J. G. Brankov
Biomed. Opt. Express 10(2) 747-760 (2019)

Transmission and fluorescence angular domain optical projection tomography of turbid media

Fartash Vasefi, Eldon Ng, Bozena Kaminska, Glenn H. Chapman, Kevin Jordan, and Jeffery J. L. Carson
Appl. Opt. 48(33) 6448-6457 (2009)

Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

Metasebya Solomon, Brian R. White, Ralph E. Nothdruft, Walter Akers, Gail Sudlow, Adam T. Eggebrecht, Samuel Achilefu, and Joseph P. Culver
Biomed. Opt. Express 2(12) 3267-3277 (2011)

Parametric imaging of the local attenuation coefficient in human axillary lymph nodes assessed using optical coherence tomography

Loretta Scolaro, Robert A. McLaughlin, Blake R. Klyen, Benjamin A. Wood, Peter D. Robbins, Christobel M. Saunders, Steven L. Jacques, and David D. Sampson
Biomed. Opt. Express 3(2) 366-379 (2012)