Add to BibSonomy
Abstract
Stimulated Raman scattering (SRS) is a powerful optical technique for probing the vibrational states of molecules in biological tissues and provides greater signal intensities than when using spontaneous Raman scattering. In this study, we examined the use of continuous wave (cw) and picosecond (ps) laser excitations to generate SRS signals in pure methanol, a carotene–methanol solution, acetone, and brain tissue samples. The cw-SRS system, which utilized two cw lasers, produced better signal-to-noise (S/N) than the conventional ps-SRS system, suggesting that the cw-SRS system is an efficient and cost-effective approach for studying SRS in complex systems like the brain. The cw-SRS approach will reduce the size of the SRS system, allowing for stimulated Raman gain/loss microscopy. In addition, we showed that there exists a resonance SRS (RSRS) effect from the carotene–methanol solution and brain tissue samples using cw laser excitations. The RSRS effect will further improve the signal-to-noise and may be utilized as an enhanced, label-free SRS microscopic tool for the study of biological tissues.
© 2020 Optical Society of America
Full Article | PDF ArticleMore Like This
Lingyan Shi, Taposh Gayen, Yury Budansky, Kerr Yoo, Jeff Secor, Thomas Harvey, George Harvey, Pavel Shumyatsky, Daniel Nolan, and Robert Alfano
Opt. Express 28(15) 21792-21804 (2020)
Andrew H. Hill, Bryce Manifold, and Dan Fu
Biomed. Opt. Express 11(2) 762-774 (2020)
Alberto Lombardini, Pascal Berto, Julien Duboisset, Esben Ravn Andresen, Sandro Heuke, Edlef Büttner, Ingo Rimke, Sébastien Vergnole, Vasyl Shinkar, Philippe de Bettignies, and Hervé Rigneault
Opt. Express 28(10) 14490-14502 (2020)