Abstract

We propose a single-beam high-resolution quantitative phase imaging method based on a spatial light modulator (SLM) and an incremental binary random sampling (IBRS) algorithm. In this method, the image of the test object presents on the image sensor through an optical microscopy system composed of an objective lens and a collimating lens. A transmittance SLM displaying a group of well-designed IBRS patterns is inserted in the optical microscopy system to modulate the object wavefront. The phase information of the object image can be quantitatively retrieved from the recorded intensities using the IBRS algorithm and the amplitude obtained directly from the diffraction intensity. The IBRS algorithm employed in our method has higher accuracy for phase retrieval compared with our previously proposed complementary random sampling algorithm, which is confirmed by simulations. Further, we demonstrate experimentally the feasibility of our method through several examples: phase imaging of immersion oil droplets with a diffraction-limited lateral resolution of 1.54 µm and a few microbiological specimens with 0.70 µm. Experimental results reveal that our proposed method provides a feasible single-beam technique for quantitative phase imaging with a high spatial resolution.

© 2020 Optical Society of America

Wavefront sensing based on a spatial light modulator and incremental binary random sampling

Ben-Yi Wang, Lu Han, Yang Yang, Qing-Yang Yue, and Cheng-Shan Guo
Opt. Lett. 42(3) 603-606 (2017)

Phase retrieval and diffractive imaging based on Babinet's principle and complementary random sampling

Zhen-Jia Cheng, Ben-Yi Wang, Yi-Yan Xie, Yu-Jie Lu, Qing-Yang Yue, and Cheng-Shan Guo
Opt. Express 23(22) 28874-28882 (2015)

Structured illumination contrast transfer function for high resolution quantitative phase imaging

Sibi Chakravarthy Shanmugavel and Yunhui Zhu
Opt. Express 31(24) 40151-40165 (2023)

Phase retrieval by random binary amplitude modulation and ptychography principle

Cheng Xu, Hui Pang, Axiu Cao, Qiling Deng, and Huajun Yang
Opt. Express 30(9) 14505-14517 (2022)

Cross-talk compensation of a spatial light modulator for iterative phase retrieval applications

Pierre Gemayel, Bruno Colicchio, Alain Dieterlen, and Pierre Ambs
Appl. Opt. 55(4) 802-810 (2016)