Abstract

The development of light-sheet fluorescence microscopy has been a revolution for developmental biology as it allows long-term imaging during embryonic development. An important reason behind the quick adoption has been the availability of open hardware alternatives. In this work, we present a robust and compact version of a light-sheet fluorescence microscope that is easy to assemble and requires little to no maintenance. An important aspect of the design is that the illumination unit consists of reflective elements, thereby reducing chromatic aberrations an order of magnitude as compared to refractive counterparts.

© 2020 Optical Society of America

Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy

Matteo Bernardello, Radoslaw J. Gora, Patrick Van Hage, Gustavo Castro-Olvera, Emilio J. Gualda, Marcel J. M. Schaaf, and Pablo Loza-Alvarez
Biomed. Opt. Express 12(10) 6205-6227 (2021)

Light-sheet fluorescence microscopy for the in vivo study of microtubule dynamics in the zebrafish embryo

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High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip

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Hard-wired lattice light-sheet microscopy for imaging of expanded samples

Anne Stockhausen, Jana Bürgers, Juan Eduardo Rodriguez-Gatica, Jens Schweihoff, Rudolf Merkel, Jens Markus Prigge, Martin Karl Schwarz, and Ulrich Kubitscheck
Opt. Express 28(10) 15587-15600 (2020)

Light-sheet microscopy: a tutorial

Omar E. Olarte, Jordi Andilla, Emilio J. Gualda, and Pablo Loza-Alvarez
Adv. Opt. Photon. 10(1) 111-179 (2018)